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PERSONAL EXPERIMENT with Wnt3a and Keratinocytes


#1

Hey all,

I’ve been a long time reader and a first time poster. I’m about to shell out almost $1,000 to do an experiment and need your inputs so I go about this the right way and not waste any time or money. I’ve been reading up a lot on all these follica and histogen experiments, and there seems to be some great promise. This is what I plan to do, please tell me if you see any faults with this and how you would recommend that I fix them:

I plan to dermabrate my scalp and wait a couple of days (not sure how many…possibly 3 days later) to create an embryonic environment and encourage stem cells to migrate. Then, I will inject universally a mixture of wnt3a and keratinocytes and watch for any results. Keeping it simple.

Do you think that I should mix in any other growth factors, or anything that would enhance beta catenin or lef with the wnt3a? Maybe add in prostaglandins like travoprost and latanoprost, or maybe a prostamide like bimatoprost?

The interesting followup question would be if any new follicles would be DHT resistant? Probably not so I’d have to get some kind of topical anti-androgen for maintenance.

Your input will be very much appreciated. Thanks!


#2

» Hey all,
»
» I’ve been a long time reader and a first time poster. I’m about to shell
» out almost $1,000 to do an experiment and need your inputs so I go about
» this the right way and not waste any time or money. I’ve been reading up a
» lot on all these follica and histogen experiments, and there seems to be
» some great promise. This is what I plan to do, please tell me if you see
» any faults with this and how you would recommend that I fix them:
»
»
» I plan to dermabrate my scalp and wait a couple of days (not sure how
» many) to create an embryonic environment and encourage stem cells to
» migrate. Then, I will inject universally a mixture of wnt3a and
» keratinocytes and watch for any results.
»
» Do you think that I should mix in any other growth factors, or anything
» that would enhance beta catenin or lef with the wnt3a? Maybe add in
» prostaglandins like travoprost and latanoprost, or maybe a
» prostamide like bimatoprost?
»
» The interesting followup question would be if any new follicles would be
» DHT resistant? Probably not so I’d have to get some kind of topical
» anti-androgen for maintenance.
»
» Your input will be very much appreciated. Thanks!

What’s your background? Any experience in the science and/or medical area?
I’ve read horror stories with self-injecting. We’re not talking vitamin b12 here. You’re taking a huge risk and although I’d love to see how it turns out, I’m totally against your experiment. Reconsider it.


#3

» What’s your background? Any experience in the science and/or medical
» area?
» I’ve read horror stories with self-injecting. We’re not talking vitamin
» b12 here. You’re taking a huge risk and although I’d love to see how it
» turns out, I’m totally against your experiment. Reconsider it.

Thanks for your concern. I have done extensive research and read through almost all the experiments involving wnt and follicular morpheogenesis. At this point, the only thing that I think that I have to worry about is benign tumors. It’s a very minimal risk (since I will be only doing one injection of the wnt mixture).

It’s come to a point where everyone is all talk and no action and I just can’t wait around for these companies and the FDA anymore. I want to do this for you all and myself. And this won’t be another five years. It will be now, with your help. So, please give any input that you think can help to move this along and I will keep you all posted with my progress.

All the best.


#4

The last time I did anything resembling wounding-based regrowth, I think I ended up possibly doing damage to the remaining hair in the area. Just a warning.

And as I said on the other thread you posted in, I think the immune suppression is necessary.

Best of luck. I would tell you this is unsafe but I think you already know that pretty well. We all benefit from every attempt including the failed ones, so thank you for that.


#5

» Hey all,
»
» I’ve been a long time reader and a first time poster. I’m about to shell
» out almost $1,000 to do an experiment and need your inputs so I go about
» this the right way and not waste any time or money. I’ve been reading up a
» lot on all these follica and histogen experiments, and there seems to be
» some great promise. This is what I plan to do, please tell me if you see
» any faults with this and how you would recommend that I fix them:
»
»
» I plan to dermabrate my scalp and wait a couple of days (not sure how
» many…possibly 3 days later) to create an embryonic environment and
» encourage stem cells to migrate. Then, I will inject universally a mixture
» of wnt3a and keratinocytes and watch for any results. Keeping
» it simple.
»
» Do you think that I should mix in any other growth factors, or anything
» that would enhance beta catenin or lef with the wnt3a? Maybe add in
» prostaglandins like travoprost and latanoprost, or maybe a
» prostamide like bimatoprost?
»
» The interesting followup question would be if any new follicles would be
» DHT resistant? Probably not so I’d have to get some kind of topical
» anti-androgen for maintenance.
»
» Your input will be very much appreciated. Thanks!

Why wnt3a? Im pretty sure wnt7a was used in the first nature article. You should check that out.


#6

» The last time I did anything resembling wounding-based regrowth, I think I
» ended up possibly doing damage to the remaining hair in the area. Just a
» warning.
»
» And as I said on the other thread you posted in, I think the immune
» suppression is necessary.
»
»
»
» Best of luck. I would tell you this is unsafe but I think you already
» know that pretty well. We all benefit from every attempt including the
» failed ones, so thank you for that.

What exactly was it that you did that led to the negative results? It would help to know so that I don’t possibly make the same mistakes. Also, many people keep telling me that this is unsafe. What about this will be unsafe, besides the very very very slight chance that a benign tumor may form?

Also, I’ve read quite a few studies where immunosupression was not performed and yet, great results were achieved. Why should this be a necessary component? If I did decide to go ahead with the immmunosupression, I assume this would not be possible to do locally. Thus, what have you guys from your experiences found to be the best way to do this with minimal side effects?


#7

» Why wnt3a? Im pretty sure wnt7a was used in the first nature article. You
» should check that out.

Both will work, but it seems from what I’ve read that wnt3a is the “preferred” method.


#8

» » Why wnt3a? Im pretty sure wnt7a was used in the first nature article.
» You
» » should check that out.
»
» Both will work, but it seems from what I’ve read that wnt3a is the
» “preferred” method.

GODSPEED


#9

» Hey all,
»
» I’ve been a long time reader and a first time poster. I’m about to shell
» out almost $1,000 to do an experiment and need your inputs so I go about
» this the right way and not waste any time or money. I’ve been reading up a
» lot on all these follica and histogen experiments, and there seems to be
» some great promise. This is what I plan to do, please tell me if you see
» any faults with this and how you would recommend that I fix them:
»
»
» I plan to dermabrate my scalp and wait a couple of days (not sure how
» many…possibly 3 days later) to create an embryonic environment and
» encourage stem cells to migrate. Then, I will inject universally a mixture
» of wnt3a and keratinocytes and watch for any results. Keeping
» it simple.
»
» Do you think that I should mix in any other growth factors, or anything
» that would enhance beta catenin or lef with the wnt3a? Maybe add in
» prostaglandins like travoprost and latanoprost, or maybe a
» prostamide like bimatoprost?
»
» The interesting followup question would be if any new follicles would be
» DHT resistant? Probably not so I’d have to get some kind of topical
» anti-androgen for maintenance.
»
» Your input will be very much appreciated. Thanks!

As promised, I’m keeping everyone updated:

Nov 16, 2009: I took before pix and dermabrated my scalp. (Not sure if I plan on posting pix).

I have wnt3a and keratinocyte (kgf) mixture in cooler ready to be injected in several days. Did not take any immuno-suppressants or add anything else to the mixture.


#10

Good luck, I hope you get some results. It sure beats everyone sitting around waiting.


#11

» Good luck, I hope you get some results. It sure beats everyone sitting
» around waiting.

agreed wish u the best keep on telling us how things go if they are good i am definitely going to be trying this. actually no matter what I’m going to do it right now though i only have $600 to my name so its just a matter of saving. i think cal was right though because a guy named benji on here said the exact same thing about suppressing the immune system. i know this benji guy is pretty on top of his game when it comes to the follica experiment.

GOOD LUCK MAN!

I HOPE IT WORKS

and… thank you for doing this it helps us all


#12

» » Good luck, I hope you get some results. It sure beats everyone sitting
» » around waiting.
»
» agreed wish u the best keep on telling us how things go if they are
» good i am definitely going to be trying this. actually no matter what I’m
» going to do it right now though i only have $600 to my name so its just a
» matter of saving. i think cal was right though because a guy named benji
» on here said the exact same thing about suppressing the immune system. i
» know this benji guy is pretty on top of his game when it comes to the
» follica experiment.
»
»
» GOOD LUCK MAN!
»
» I HOPE IT WORKS
»
» and… thank you for doing this it helps us all

Dude… seriously… best of luck to you…


#13

» » » Good luck, I hope you get some results. It sure beats everyone sitting
» » » around waiting.
» »
» » agreed wish u the best keep on telling us how things go if they
» are
» » good i am definitely going to be trying this. actually no matter what
» I’m
» » going to do it right now though i only have $600 to my name so its just
» a
» » matter of saving. i think cal was right though because a guy named
» benji
» » on here said the exact same thing about suppressing the immune system.
» i
» » know this benji guy is pretty on top of his game when it comes to the
» » follica experiment.
» »
» »
» » GOOD LUCK MAN!
» »
» » I HOPE IT WORKS
» »
» » and… thank you for doing this it helps us all
»
»
» Dude… seriously… best of luck to you…

Yeah, I thought in the description they suggested the possible use of Hydrocortisone or Betamethasone or some such…might be worth bearing in mind.


#14

» » Hey all,
» »
» » I’ve been a long time reader and a first time poster. I’m about to
» shell
» » out almost $1,000 to do an experiment and need your inputs so I go
» about
» » this the right way and not waste any time or money. I’ve been reading up
» a
» » lot on all these follica and histogen experiments, and there seems to
» be
» » some great promise. This is what I plan to do, please tell me if you
» see
» » any faults with this and how you would recommend that I fix them:
» »
» »
» » I plan to dermabrate my scalp and wait a couple of days (not sure how
» » many…possibly 3 days later) to create an embryonic environment and
» » encourage stem cells to migrate. Then, I will inject universally a
» mixture
» » of wnt3a and keratinocytes and watch for any results.
» Keeping
» » it simple.
» »
» » Do you think that I should mix in any other growth factors, or anything
» » that would enhance beta catenin or lef with the wnt3a? Maybe add in
» » prostaglandins like travoprost and latanoprost, or maybe
» a
» » prostamide like bimatoprost?
» »
» » The interesting followup question would be if any new follicles would
» be
» » DHT resistant? Probably not so I’d have to get some kind of topical
» » anti-androgen for maintenance.
» »
» » Your input will be very much appreciated. Thanks!
»
» As promised, I’m keeping everyone updated:
»
» Nov 16, 2009: I took before pix and dermabrated my scalp. (Not sure if I
» plan on posting pix).
»
» I have wnt3a and keratinocyte (kgf) mixture in cooler ready to be injected
» in several days. Did not take any immuno-suppressants or add anything else
» to the mixture.

I hope you wont miss the “embryonic window”. Too soon or to late and it is all for nothing. I would wait until the scab falls off but you do what u think will work best. Good luck.


#15

»
» I hope you wont miss the “embryonic window”. Too soon or to late and it is
» all for nothing. I would wait until the scab falls off but you do what u
» think will work best. Good luck.

Thanks. I am considering doing three injections on three consecutive days starting tomorrow to ensure that I do not miss the so called “embryonic window”. I have more than enough of the solution to inject so I may as well. The only issue / risk with this is the possible tumors that could form from unhalted signals, but my understanding is that the signals need to go on for months non-stop to cause any benign tumors. I’m going to contact some people just to make sure and I’ll keep you guys updated on my decision.

WORST NIGHTMARE: http://www.pathconsultddx.com/images/S1559867506701899/gr1-sml.jpg


#16

Are you planning on wounding the injection area at all? Or just straight to injections?

The reason I think the immune suppression is necessary is several things, but mainly just because nobody is having any luck growing anything without it.

There have been a bunch of guys trying one thing or another, including several attempts that have included the main factors that are believed to cause this miraculous wound-based regrowth that has happened accidentally on the cancer patients. Whether they got it perfectly or not, I would have expected at least a little bit of growth or signs of progress from them if there was a way. Given what has really happened with these attempts I find it more realistic to think that the immune system (which is clearly relevant to hair thinning/growth as a whole) is smashing down the regeneration process.

For what it’s worth, this failed me recently:

I took some oral Leflunomide (Genfilitnib-like effects) while using a topical cortisone cream on the area for a few weeks. This should have been fairly similar to the cancer patients who got accidental regrowth from Genfilitnib usage.

What concerns me is that I’m fairly sure the areas I tested are worse off than before I started. They were in areas of thin hairline growth where I wounded and applied the topical cortisone cream. Afterward, now the surrounding hairline areas are still growing about like they were before the attempt, but the lost hair in the wounded areas is showing no signs of coming back. It’s been a good 4 months since the original wound date now.


#17

very fast post here i gotta go do something but

call this ignorance but what i dont understand is wewillwin is using Wnt3a and Keratinocy i thought it was Genfilitnib(taken orally or topically) and things like milk thistle blah blah blah im not going to list them all but were outlined in other posts. im so confused he also says hes going to inject the area (with a needle???) i thought it was orally and topically applied. err owell i hope it works and i think cal is right you have to find a way to suppress the immune system.


#18

» very fast post here i gotta go do something but
»
» call this ignorance but what i dont understand is wewillwin is using Wnt3a
» and Keratinocy i thought it was Genfilitnib(taken orally or topically) and
» things like milk thistle blah blah blah im not going to list them all but
» were outlined in other posts. im so confused he also says hes going to
» inject the area (with a needle???) i thought it was orally and topically
» applied. err owell i hope it works and i think cal is right you have to
» find a way to suppress the immune system.

Check your post about eyebrows. I found a HT doc that does eyebrow transplants.


#19

» very fast post here i gotta go do something but
»
» call this ignorance but what i dont understand is wewillwin is using Wnt3a
» and Keratinocy i thought it was Genfilitnib(taken orally or topically) and
» things like milk thistle blah blah blah im not going to list them all but
» were outlined in other posts. im so confused he also says hes going to
» inject the area (with a needle???) i thought it was orally and topically
» applied. err owell i hope it works and i think cal is right you have to
» find a way to suppress the immune system.

Sorry, I should have sourced the basis of my approach. I don’t have much time to get into it right now, but please read through this patent:

http://www.google.com/patents?id=V6AUAAAAEBAJ&printsec=abstract&zoom=4&source=gbs_overview_r&cad=0#v=onepage&q=&f=false

Also, I am using a syringe to inject the solution. It’s a very superficial injection using a microsyringe. I do not believe that immunosuppression will be necessary for this. I have already wounded / dermabrated and plan on only doing one time injection all over my scalp in the morning. Although I’ve consulted with several people who have told me that a few injections should be safe over several days, I decided to just do one and see how it goes. Also, I decided not to post any pictures for some personal reasons, but I’ll keep you guys updated as this goes.


#20

» » very fast post here i gotta go do something but
» »
» » call this ignorance but what i dont understand is wewillwin is using
» Wnt3a
» » and Keratinocy i thought it was Genfilitnib(taken orally or topically)
» and
» » things like milk thistle blah blah blah im not going to list them all
» but
» » were outlined in other posts. im so confused he also says hes going to
» » inject the area (with a needle???) i thought it was orally and
» topically
» » applied. err owell i hope it works and i think cal is right you have
» to
» » find a way to suppress the immune system.
»
»
» Sorry, I should have sourced the basis of my approach. I don’t have much
» time to get into it right now, but please read through this patent:
»
» http://www.google.com/patents?id=V6AUAAAAEBAJ&printsec=abstract&zoom=4&source=gbs_overview_r&cad=0#v=onepage&q=&f=false
»
» Also, I am using a syringe to inject the solution. It’s a very superficial
» injection using a microsyringe. I do not believe that immunosuppression
» will be necessary for this. I have already wounded / dermabrated and plan
» on only doing one time injection all over my scalp in the morning.
» Although I’ve consulted with several people who have told me that a few
» injections should be safe over several days, I decided to just do one and
» see how it goes. Also, I decided not to post any pictures for some personal
» reasons, but I’ll keep you guys updated as this goes.

Please just promise us (pinky swear) :slight_smile: u will tell us honestly if it works or not and the details