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Topical green tea................massive reduction in sebum: Study


#1
  1. Catechin Inhibition of Human Forehead Sebum Production

Twenty mg of (-)epigallocatechin gallate (EGCG) in 0.2 ml 70% ethanol was applied to the left forehead twice a day for 6 days (FIG. 25). The L/R ratio decrease from 1.20±0.02 to 0.71±0.04 during this period. After the EGCG treatment was stopped, the L/R ratio gradually recovered to 1.19±0.02 within 16 days. Subsequent treatment of the left forehead with 20 mg (-)epicatechin in 0.2 ml 70% ethanol twice a day for 6 days reduced the L/R ratio to 1.02±0.1. After the application was stopped, the L/R ratio gradually increased to the normal value of 1.21±0.01 in 10 days. Clearly EGCG was more effective in reducing the sebum production from forehead than (-)epicatechin.

http://www.freepatentsonline.com/5605929.html


#2

» 2. Catechin Inhibition of Human Forehead Sebum Production
»
» Twenty mg of (-)epigallocatechin gallate (EGCG) in 0.2 ml 70% ethanol
» was applied to the left forehead twice a day for 6 days (FIG. 25). The L/R
» ratio decrease from 1.20±0.02 to 0.71±0.04 during this period. After the
» EGCG treatment was stopped, the L/R ratio gradually recovered to 1.19±0.02
» within 16 days.
Subsequent treatment of the left forehead with 20 mg
» (-)epicatechin in 0.2 ml 70% ethanol twice a day for 6 days reduced the
» L/R ratio to 1.02±0.1. After the application was stopped, the L/R ratio
» gradually increased to the normal value of 1.21±0.01 in 10 days. Clearly
» EGCG was more effective in reducing the sebum production from forehead
» than (-)epicatechin.
»
»
»
» http://www.freepatentsonline.com/5605929.html

Damn! I drink enough of the stuff. Now I have to put it on my head too?


#3

there is a big diffence between topical application (especially of natural compounds) and internal digestion of them. The human digestive system breaks down many moleculed in digestion or alters them, rendering their topical and internal effect entirely different.

Curcumin, which in tests with hamster flank organs seems to be profoundly anti-androgenic, obviously isn’t like eating internal flutamide when you have Indian Curry spice with a meal. Green tea catechin ECGC is apparently much the same.


#4

I’ve read that oral EGCG is poorly absorbed, which probably explains why drinking green tea hasn’t been accepted for centuries as being some sort of “home remedy” for oily skin. I think if it really did do anything in the simple form of green tea beverage, we would have ALL known about by now, and its use as an “antiandrogen” of sorts (I use the term loosely, of course) would already be well-known to medical science.

Its topical use on the skin/scalp is a separate issue, obviously.

.


#5

adding citris (orange/lemon/lime) to gr tea helps in the absortion of EGCG

I’ve read that oral EGCG is poorly absorbed, which probably explains why
» drinking green tea hasn’t been accepted for centuries as being some sort
» of “home remedy” for oily skin. I think if it really did do anything in
» the simple form of green tea beverage, we would have ALL known about by
» now, and its use as an “antiandrogen” of sorts (I use the term loosely, of
» course) would already be well-known to medical science.
»
» Its topical use on the skin/scalp is a separate issue, obviously.
»
» .


#6

» adding citris (orange/lemon/lime) to gr tea helps in the absortion of EGCG

How do you know that?

.


#7

» » adding citris (orange/lemon/lime) to gr tea helps in the absortion of
» EGCG
»
» How do you know that?
»
» .

I doubt it would have any topical effect, but I was able to google some articles concerning citrus juices effect on green tea digestion and absorption…I’ll paste one (there were quite a few others0:

"Green tea up to 5 times as healthy when citrus juice added
Last Updated: Wednesday, November 14, 2007 | 3:31 PM ET
CBC News
You’ve likely seen the studies that argue green tea is a powerful brew of cancer-fighting antioxidants.

But new research shows that although green tea is high in catechins — antioxidants that have been shown to reduce the risk of chronic diseases like cancer and cardiovascular disorders — the amount of these substances that are available for absorption by the body are lower when they’re consumed solely in tea.

The researchers believe that citrus juices and vitamin C protect catechins from oxidative damage in the intestine.
(CBC) Under 20 per cent of catechins, which include EGC, EGCG, EC and ECG, remain after digestion, say the authors.

Scientists have discovered that mixing green tea with other substances, such as citrus juices, vitamin C and even soy milk and rice milk, increases the amount of the antioxidants that can be absorbed by the body.

The study is published in the November issue of Molecular Nutrition and Food Research.

According to Mario Ferruzzi, assistant professor of food science at Purdue University in Indiana, and the study’s lead author, catechins break down in non-acidic environments, such as the intestines, leaving less than 20 per cent of the antioxidants to be absorbed after digestion takes place.

“We have to address this fact if we want to improve bodily absorption,” he said in a release issued Tuesday.

Ferruzzi and his colleagues experimented by adding a variety of substances to green tea and then simulated digestion.

Citrus juices, including from limes, grapefruits, lemons and oranges, increased the levels of catechins by more than five times, with an 81 to 98 per cent recovery for EGC, 56 to 75 per cent for EGCG, 86 to 95 per cent for EC and 30 to 55 per cent for ECG.

Lemon juice was the most effective at preserving catechins in green tea, the study found.

Adding 30 milligrams of vitamin C to the green tea, increased recovery of the antioxidant ECG, EGCG, EC and ECG to 74 per cent, 54 per cent, 82 per cent and 45 per cent respectively.

The researchers believe that the juices and vitamin C protect the catechins from oxidative damage in the intestine. Juices also have fibre and polysaccharides that can protect the antioxidants, they theorize.

Green tea with 50 per cent cow’s milk increased the amount of catechins to 52 per cent, soy milk raised it to 55 per cent and rice milk raised it to 69 per cent."

Me again…the thing is I dont think I’D WANT to absorb much ECGC internally if it led to a blockage of a percentage of my bodies androgen receptors. I took fluatmide for about five days one (topically) and felt lousy.

BTW—Liang and Laou found some things in black tea also inhibited alpha five reductase topically in patents that mention their research.


#8

Ive cut and pasted from the patent (a very long patent indeed, geesh):
http://www.patentstorm.us/patents/6696484-description.html

  1. Green Tea Catechins

                          TABLE 1
    

    Inhibition of 5 α-reductase isozymes by green tea catechinsa
    5 α-Reductase
    Cell-Free assay IC50 (µM) Whole-cell assay IC50
    (µM)
    Catechin Type 1 Type 2 Type 1 Type 2
    EC >100 (14) >100 (4) >100 (0) >100 (1)
    EGC >100 (15) >100 (3) >100 (15) >100 (1)
    ECG 11 (100) 69 (83) >100 (0) >100 (0)
    EGCG 15 (99) 74 (74) >100 (6) >100 (0)
    a IC50 : concentration (µM) of compound producing 50%
    inhibition of 5 α-reductase activity. Values in parentheses are
    percent inhibition of 5 α-reductase activity in the presence of 100
    µM concentration of the indicated compound.

Here is another:

  1. Flavonoids

A variety of naturally occurring flavonoids with structures related to the tea catechins were also tested (FIG. 1, Table 3).

                         TABLE 3
Inhibition of 5 α-reductase isozymes by various natural
 flavonoidsa
                           5 α-Reductase
              Cell-Free assay IC50 (µM) Whole-cell assay IC50
 (µM)
Polyphenol    Type 1      Type 2      Type 1      Type 2
Myricetin       23 (96)   >100 (31) >100 (11) >100 (11)
Quercitin       23 (96)   >100 (14) >100 (15) >100 (29)
Baicalein       29 (79)     99 (51)   >100 (24) >100 (4)
Fisetin         57 (97)   >100 (4) >100 (42) >100 (27)
Biochanin A   >100 (50)   17 (74)     64 (64)     5 (93)
Daidzein      >100 (3)   29 (69)     10 (13)     7 (89)
Kaempferol    >100 (22)   12 (62)     79 (60)     20 (85)
Flavone       >100 (20) >100 (-52) NDb    ND
Genistein     >100 (16)   23 (76)   >100 (22)   20 (89)
Morin         >100 (6) >100 (33) ND          ND
Alpha-        >100 (6) >100 (-13) ND          ND
napthoflavone
Taxifolin     >100 (5) >100 (5) ND          ND
Beta-         >100 (3) >100 (4) ND          ND
napthoflavone
Chrysin       >100 (2) >100 (1) ND          ND
Rutin         >100 (4) >100 (0) ND          ND

Another:

                         TABLE 4
Inhibition of 5 α-reductase by compounds containing catecholsa
                             5 α-Reductase
                                            Whole-cell
                  Cell-free assay IC50 (µM) assay IC50 (µM)
Catechol          Type 1    Type 2      Type 1      Type 2
Anthrarobon          4 (99)    50 (97)     6 (91)   >100 (31)
Bromopyrogallol      7 (98)    84 (58)  NDb    ND
red
Gossypol             7 (99)    21 (99)     7 (100)     6 (99)
Pyrogallol red       15 (97) >100 (27) ND          ND
Nordihydro-          19 (99)    50 (80)     19 (99)     22 (99)
guaiaretic acid
Caffeic acid         26 (97) >100 (36)    8 (99)      7 (98)
phenethyl ester
Octyl gallate        27 (99)    58 (90)     7 (99)      18 (94)
Purpurogallin        30 (81) >100 (31) ND          ND
Hydroxydopamine      42 (69) >100 (41) ND          ND
Dodecyl gallate      43 (88) >100 (36)    3 (99)      7 (98)
Pyrocatechol violet    48 (85)   100 (47)  ND          ND
Pyrogallol           70 (60) >100 (28) >100 (7) >100 (15)
Hematoxylin          83 (59) >100 (38) ND          ND
HZIV-82           >100 (43) >100 (0)    3 (79)   >100 (15)
Cnc               >100 (42) >100 (-75) ND          ND
HZIV 90           >100 (23) >100 (13) >100 (34) >100 (14)
Caffeic acid      >100 (13) >100 (8) ND          ND
HZIV 275          >100 (10) >100 (6) ND          ND
Esculetin         >100 (7) >100 (13) ND          ND
Ellagic acid      >100 (7) >100 (9) ND          ND
Catechol          >100 (5) >100 (0) >100 (9) >100 (3)
Methyl gallate    >100 (5) >100 (3) >100 (0) >100 (0)
Propyl gallate    >100 (0) >100 (0) >100 (5) >100 (0)
Fraxetin          >100 (2) >100 (8) ND          ND

Curcumoids:

TABLE 5
Inhibition of 5 α-reductase isozymes by curcumin and related
compoundsa
5 α-Reductase
Whole-cell
Cell-Free assay IC50 (µM) assay IC50 (µM)
Compound Type 1 Type 2 Type 1 Type 2
Curcumin 3 (95) 5 (87) 9 (99) 7 (99)
Tetrahydrocurcumin 80 (56) 29 (73) NDb ND
Demethoxy- >100 (23) >100 (42) ND ND
tetrahydrocurcumin
4-hydroxy-3- >100 (10) >100 (-60) ND ND
methoxy-
cinnamaldehyde
Coniferol >100 (10) 100 (49) ND ND
4-(4-hydroxy-3- >100 (3) >100 (4) ND ND
methoxyphenol)-3-
buten-2-one
Ferulic Acid >100 (0) >100 (18) ND ND
Capsaicin >100 (0) >100 (8) ND ND
Eugenol >100 (0) 100 (50) ND ND

                         TABLE 1
Inhibition of 5 α-reductase isozymes by green tea catechinsa
                           5 α-Reductase
              Cell-Free assay IC50 (µM) Whole-cell assay IC50
 (µM)
Catechin      Type 1      Type 2      Type 1      Type 2
EC            >100 (14) >100 (4) >100 (0) >100 (1)
EGC           >100 (15) >100 (3) >100 (15) >100 (1)
ECG            11 (100)    69 (83)    >100 (0) >100 (0)
EGCG           15 (99)     74 (74)    >100 (6) >100 (0)

                         TABLE 2
Inhibition of 5 α-reductase by various EGC derivativesa
                            5 α-Reductase
                                         Whole-cell assay
                Cell-free assay IC50 (µM)  IC50 (µM)
EGC derivatives Type 1      Type 2      Type 1      Type 2
 1. EGCG           12 (99)     73 (76)  >100 (11) >100 (5)
 2. HZIV 160       29 (99)     76 (96)     7 (99)      8 (98)
 3. HZIV 134       20 (99)     67 (94)  NDb    ND
 4. HZIV 92        23 (98)  >100 (45)    64 (94)     80 (62)
 5. HZIV 120       23 (99)     66 (97)     49 (97)     57 (96)
 6. HZIV 142       25 (97)     63 (93)     8 (99)      14 (98)
 7. HZIV 68        29 (93)     99 (51)  >100 (40) >100 (34)
 8. HZIV 75        29 (97)  >100 (21)    43 (83)     62 (72)
 9. HZIV 166       30 (98)     78 (74)     58 (89)     72 (83)
10. HZIV 63       311 (94)  >100 (20) >100 (12) >100 (7)
11. HZIV 169       47 (90)  >100 (39) >100 (10) >100 (0)
12. HZIV 74        48 (85)  >100 (24) ND          ND
13. HZIV 144       49 (88)  >100 (38) >100 (12) >100 (7)
14. HZIV 168       49 (98)     73 (92)     28 (93)     41 (94)
15. HZIV 166       59 (95)     71 (84)     58 (89)     72 (83)
16. EGC            62 (61)  >100 (30) >100 (15) >100 (1)
17. HZIV 107       98 (52)  >100 (39) >100 (23) >100 (2)
18. HZIV 145    >100 (35) >100 (8) >100 (8) >100 (9)
19. HZIV 148    >100 (31) >100 (0)    42 (90)     74 (81)
20. HZIV 109    >100 (17) >100 (0) ND          ND
a IC50 : concentration (µM) of compound producing 50%
 



                         TABLE 4
Inhibition of 5 α-reductase by compounds containing catecholsa
                             5 α-Reductase
                                            Whole-cell
                  Cell-free assay IC50 (µM) assay IC50 (µM)
Catechol          Type 1    Type 2      Type 1      Type 2
Anthrarobon          4 (99)    50 (97)     6 (91)   >100 (31)
Bromopyrogallol      7 (98)    84 (58)  NDb    ND
red
Gossypol             7 (99)    21 (99)     7 (100)     6 (99)
Pyrogallol red       15 (97) >100 (27) ND          ND
Nordihydro-          19 (99)    50 (80)     19 (99)     22 (99)
guaiaretic acid
Caffeic acid         26 (97) >100 (36)    8 (99)      7 (98)
phenethyl ester
Octyl gallate        27 (99)    58 (90)     7 (99)      18 (94)
Purpurogallin        30 (81) >100 (31) ND          ND
Hydroxydopamine      42 (69) >100 (41) ND          ND
Dodecyl gallate      43 (88) >100 (36)    3 (99)      7 (98)
Pyrocatechol violet    48 (85)   100 (47)  ND          ND
Pyrogallol           70 (60) >100 (28) >100 (7) >100 (15)
Hematoxylin          83 (59) >100 (38) ND          ND
HZIV-82           >100 (43) >100 (0)    3 (79)   >100 (15)
Cnc               >100 (42) >100 (-75) ND          ND
HZIV 90           >100 (23) >100 (13) >100 (34) >100 (14)
Caffeic acid      >100 (13) >100 (8) ND          ND
HZIV 275          >100 (10) >100 (6) ND          ND
Esculetin         >100 (7) >100 (13) ND          ND
Ellagic acid      >100 (7) >100 (9) ND          ND
Catechol          >100 (5) >100 (0) >100 (9) >100 (3)
Methyl gallate    >100 (5) >100 (3) >100 (0) >100 (0)
Propyl gallate    >100 (0) >100 (0) >100 (5) >100 (0)
Fraxetin          >100 (2) >100 (8) ND          ND
  1. Quinones

A variety of quinones were tested for activity against 5α-reductase (Table 6, FIG. 4).

                         TABLE 6
Inhibition of 5 α-reductase isozymes by quinonesa
                             5 α-Reductase
                        Cell-Free           Whole-cell
                   assay IC50 (µM)  assay IC50 (µM)
Quinone            Type 1      Type 2    Type 1    Type 2
Purpurin              2 (95)   >100 (20) NDb  ND
Alizarin              3 (95)   >100 (54)    6 (75) >100 (27)
Anthrarobin           4 (99)      50 (97)    6 (91) >100 (31)
Menadione             6 (77)      5 (81)    51 (82)    79 (62)
Coenzyme q            12 (77)     22 (81) ND        ND
2,5-                  15 (78)     17 (97) ND        ND
dichloroindophenol
Alizarin red S        30 (91)  >100 (8) >100 (22) >100 (1)
Anthrarufin           40 (67)  >100 (13) ND        ND
Anthraflavic acid  >100 (27) >100 (22) ND        ND
Quinizarin         >100 (26) >100 (7) ND        ND
Lapachol           >100 (30) >100 (9) ND        ND
t-                 >100 (19) >100 (4) ND        ND
butylhydroxyquinone
Anthraquinone      >100 (6) >100 (9) ND        ND




                    TABLE 9
Effects of green tea catechin derivatives and fatty acids, on testosterone-
stimulated growth of the pigmented macules of hamster flank organs.
                           Pigmented
                          macule area  Inhibition
Treatment                 (mm2)   (%)a   P-valueb
T (control)              17.40 ± 1.93      --
T + myristoleic acid (1 mg) 4.63 ± 0.66     73      <0.05
T + EGC-myristoleate (1 mg) 5.00 ± 0.42     71      <0.05
T + γ-linolenic acid (1 mg) 3.15 ± 0.42     82      <0.05
T + EGC-γ-linoleneate acid 5.50 ± 0.56     68      <0.05
(1 mg)
T + EGC (1 mg)           5.50 ± 0.65     68      <0.05
T + EGCG (1 mg)          6.25 ± 0.82     64      <0.05
a Percent decrease in area of macules treated with tea catechins,
 fatty acid or catechin derivatives compared to the area of
 androgen-treated macules
b Mean area of androgen-treated vs catechin-, fatty acid- or catechin
 derivative-treated macules; one-way ANOVA

.

                         TABLE 10
Effects of alizarin and curcumin on testosterone- or DHT-stimulated
 growth of the pigmented macules of hamster flank organs.
                                Pigmented
                               macule area Inhibition   P-
Experiment  Treatment          (mm2)  (%)a  valueb
     I      T (control)        18.65 ± 0.69    --
            T + alizarin (1 mg)  2.40 ± 0.47    87     <0.05
            T + curcumin (1 mg)  2.40 ± 0.65    87     <0.05
    II      DHT (control)      13.67 ± 0.96    --
            DHT + alizarin (1 mg) 10.00 ± 0.43    27     <0.05
            DHT + curcumin      9.50 ± 0.21    31     <0.05
            (1 mg)
a Percent decrease in area of macules treated with alizarin or
 curcumin compared to androgen-treated macules
b Mean area of androgen-treated vs. alizarin- or curcumin-treated
 macules; one-way ANOVA

#9

Bryan,

I highlighted the things that in a whole-cell assay jumped out at me for strong inhibition of alpha five type one and type two:

Biochanin A >100 (50) 17 (74) 64 (64) 5 (93)
Daidzein >100 (3) 29 (69) 10 (13) 7 (89)
Kaempferol >100 (22) 12 (62) 79 (60) 20 (85)
Flavone >100 (20) >100 (-52) NDb ND
Genistein >100 (16) 23 (76) >100 (22) 20 (89)

Im looking around for where I can find these things topically. I think Biochanin A is in beer as well as genistein. Kaempferol is something “new” to me. These things are almost as powerful as curcumin was topically.

Aha!

Titre du document / Document title
Determination of flavonols in green and black tea leaves and green tea infusions by high-performance liquid chromatography
Auteur(s) / Author(s)
HUAFU WANG (1) ; HELLIWELL Keith (1) ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
(1) R&D Department, William Ransom and Son plc, Hitchin, Herts SG5 ILY, ROYAUME-UNI

Résumé / Abstract
Tea flavonols are potent antioxidants and make up 2-3% of the water-soluble solids from tea leaves. In this paper, the conditions necessary for hydrolysing and analysing flavonols in tea leaves and tea infusions are optimised and an isocratic elution system for the determination of the hydrolysed flavonols by high-performance liquid chromotography is presented. Aqueous ethanol was selected as the best solution for hydrolysing flavonoids in tea leaves. The contents of flavonols on a dry weight base in green tea leaves ranged from 0.83-1.59, 1.79-4.05, and 1.56 3.31 g/kg, and in black tea leaves from 0.24-0.52, 1.04-3.03, and 1.72-2.31 g/kg for myricetin, quercetin, and kaempferol, respectively. It was observed that the particle size of ground tea leaves significantly influenced the yield of flavonols. The contents of flavonols in different green tea infusions are given.

Kaempferol is in black and green tea…

Red Clover seems to be the richest source of Biochanin A…a thought


#10

[edit] Biological importance
Caffeic acid and its derivative caffeic acid phenethyl ester (CAPE) are produced in many plants including: pears, basil, thyme, verbena, tarragon, oregano, wood betony, burning bush, turmeric, dandelion, yarrow, horsetail, rosemary, hawthorn and coffee. The amount of caffeic acid is strongly dependent on the plant species.[

Bryan, did you note that the whole-cell assay inhibition in type one and type two alpha five reductase was 99% and 98% with this stuff???
Caffeic acid 26 (97) >100 (36) 8 (99) 7 (98)

Remember that jazz about a coffee-bean extract being used as a topical by some company? This is almost certainly what they were up to.

“Some of the best sources of caffeic acid are white grapes, white wine, olives, olive oil, spinach, cabbage, asparagus, and coffee (Free Radic. Biol. Med. 1996;20:933-56”


#11

» 2. Catechin Inhibition of Human Forehead Sebum Production
»
» Twenty mg of (-)epigallocatechin gallate (EGCG) in 0.2 ml 70% ethanol
» was applied to the left forehead twice a day for 6 days (FIG. 25). The L/R
» ratio decrease from 1.20±0.02 to 0.71±0.04 during this period. After the
» EGCG treatment was stopped, the L/R ratio gradually recovered to 1.19±0.02
» within 16 days.
Subsequent treatment of the left forehead with 20 mg
» (-)epicatechin in 0.2 ml 70% ethanol twice a day for 6 days reduced the
» L/R ratio to 1.02±0.1. After the application was stopped, the L/R ratio
» gradually increased to the normal value of 1.21±0.01 in 10 days. Clearly
» EGCG was more effective in reducing the sebum production from forehead
» than (-)epicatechin.
»
»
»
» http://www.freepatentsonline.com/5605929.html

do you currently have a regimen of supplements or topicals or internals, if so what is it, can you post in your signature

i would be interested in knowing

green tea is an excellent 5 AR inhibitor,


#12

»

Elsom also uses the coffee bean extract. It seems Dr. Y mentioned caffeic acid with some of the other ingreds as well.


#13