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This beats your HM studies, you want pics here they are


#1

http://www.hairsite.com/hair-loss/forum_entry-id-25116.html

Ketoconazole (KCZ), an imidazole anti-fungal agent, is known to be effective for the treatment of seborrheic dermatitis and dandruff. In addition, 2% KCZ shampoo was found to improve hair density and the size and proportion of anagen follicles in androgenetic alopecia (AGA) [1] and, in combination with finasteride, to have an additive effect for AGA [2]. Recently, it has been reported that topical application of KCZ stimulates hair growth in C3H/HeN mice [3]. However, whether topical KCZ is effective enough to improve the clinical appearance of AGA is not yet clear. We therefore carried out an open trial of topical 2% KCZ lotion (Nizoral®) in combination with shampoos. Furthermore, to identify the mechanism, which can explain the clinical effect of KCZ on AGA, we performed transient transfection assays using CV-1 cells transfected with androgen receptors (AR).

The six Japanese males from 23 to 51 years old were enrolled in this study with their written informed consent. They presented with grade II vertex to IVa AGA according to the Hamilton–Norwood classification [4]. The subjects applied topical 2% KCZ lotion (Nizoral®) almost every day during or immediately after hair washing with their own unmedicated shampoos. When they revisited our clinic every several months, clinical pictures were obtained to determine the efficacy of the treatment. Two of the men, one 23 years old with grade II vertex and the other 25 years old with Va AGA, showed remarkable hair regrowth after 6 and 10 months, respectively (Fig. 1). The 23-year-old male stopped using KCZ and 3 months later hair loss recurrence on the vertex was noted (Fig. 1c). When he started using KCZ again during shampooing, hairs on the vertex grew again after 3 months (Fig. 1d). These findings constitute evidence of the clinical efficacy of KCZ for AGA. A 41-year-old male showed a slight increase in vertex hair growth after 1 year. Other three of the men, 31, 38 and 51 years old did not show significant improvement. These findings suggest that topical KCZ with shampoo can be effective for some males with AGA.

Fig. 1. A 23-year-old Japanese man who used 2% ketoconazole (KCZ) lotion during shampooing everyday. Six months later, hair regrowth was attained (b) in comparison with the pre-treatment condition (a). Suspension of use for 3 months, however, caused recurrent hair loss ©. Renewed use of KCZ induced renewed growth of vertex hair (d). A 25-year-old Japanese man with AGA (e) applied 2% KCZ lotion immediately after shampooing everyday. Ten months later, hair regrowth was apparent (f).

To identify the mechanism, which can explain the effect of KCZ on AGA, we performed transient transfection assays using an androgen-responsive synthetic promoter for CV-1 cells transfected with AR. At 50–70% confluency in a 24-well plate, the CV-1 cells, maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum, were transfected with the transfection reagent Fugene-6 (Roche Diagnostics Corp., Indianapolis, IN, USA) according to the manufacturer’s instructions. For luciferase assays, we transfected 0.1μg of the reporter plasmid, pGL2-GRE3-bG-luc [5], 12.5ng of pCI-neo-BamX-AR(Gly23) [5] and 0.2μg of the pRL-CMV vector, the Renilla luciferase control reporter vector driven by the CMV immediate-early enhancer/promoter, as the internal controls. At 24h after transfection, we added fresh DMEM supplemented with 10% charcoal-treated fetal bovine serum with methyltrienolone (R1881, a stable synthetic androgen) or ethanol as a mock vehicle. The cells were also treated with KCZ (Janssen, L.P., Titusville, NJ) or ethanol as a mock vehicle. At 48h after transfection, the cells were harvested for luciferase assays. Luciferase activities were measured with a luminometer using the Dual-Luciferase™ reporter assay system (Promega, Madison, WI). The results were summarized from three independent sets of transfections and presented as mean±S.D.; statistical significance was tested with Student’s t-test. The results demonstrated that 10 or 20μg/ml KCZ reduced luciferase activity to 67.5% (p<0.01) or 49.9% (p<0.03), respectively, reflecting its suppressive action on AR (Fig. 2). This finding suggests that KCZ improves AGA through the suppression of AR activity.

Fig. 2. KCZ effect on R1881-induced AR transcactivity in CV-1 cells transfected with AR. We used the transfection reagent Fugene-6 (Roche Diagnostics Corp., Indianapolis, IN, USA) to transfect 0.1μg of the reporter plasmid, pGL2-GRE3-bG-luc, 12.5ng of pCI-neo-BamX-AR(Gly23) and 0.2μg of pRL-CMV vector (lanes 1–6) into CV-1 cells cultured at 50–70% confluency in a 24-well plate. The infected cells were treated with 10−9M R1881 (lanes 2–6) or ethanol as a mock vehicle (lane 1). After overnight incubation, KCZ at the indicated concentration was added to the cultures. The activities of the various reporter genes were compared with the luciferase activity in the presence of R1881 and the absence of KCZ (lane 2).

Dermal papilla cells are the main targets for androgen in hair follicles, as evidenced by immunohistochemistry [6] and reporter assays [7] for the detection of AR. Deep penetration of KCZ into dermal papilla is therefore necessary to realize the suppressive action on androgen in vivo. The use of KCZ in combination with detergent containing shampoos in this study may enhance KCZ penetration. On the other hand, a recent study demonstrated that KCZ stimulates hair growth in mice [3], suggesting that its effect on hair is androgen independent. To summarize, KCZ may exert its effect on AGA in both an androgen-dependent and -independent manner.

References
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[1]. [1]Pierard-Franchimont C, De Doncker P, Cauwenbergh G, Pierard GE. Ketoconazole shampoo: effect of long-term use in androgenic alopecia. Dermatology. 1998;196:474–477. MEDLINE | CrossRef

[2]. [2]Khandpur S, Suman M, Reddy BS. Comparative efficacy of various treatment regimens for androgenetic alopecia in men. J Dermatol. 2002;29:489–498. MEDLINE

[3]. [3]Jiang J, Tsuboi R, Kojima Y, Ogawa H. Topical application of ketoconazole stimulates hair growth in C3H/HeN mice. J Dermatol. 2005;32:243–247. MEDLINE

[4]. [4]Norwood OT. Male pattern baldness: classification and incidence. South Med J. 1975;68:1359–1365. MEDLINE

[5]. [5]Inui S, Nakao T, Itami S. Modulation of androgen receptor transcriptional activity by anti-acne reagents. J Dermatol Sci. 2004;36:97–101. Abstract | Full Text | Full-Text PDF (135 KB) | MEDLINE | CrossRef

[6]. [6]Itami S, Kurata S, Sonoda T, Takayasu S. Interaction between dermal papilla cells and follicular epithelial cells in vitro: effect of androgen. Br J Dermatol. 1995;132:527–532. MEDLINE

[7]. [7]Inui S, Itami S, Pan HJ, Chang C. Lack of androgen transcriptional activity in human keratinocytes. J Dermatol Sci. 2000;23:87–92. Abstract | Full Text | Full-Text PDF (106 KB) | MEDLINE | CrossRef


#2

I’ve hardly looked into Nizoral, it seems like it can have an AMAZING effect on some people. Do most people just use the shampoo or do a lot of people just rub it into the scalp afterward like these men did for the study?


#3

The improvement in the last guy is remarkable.

Is anyone trying this?


#4

» The improvement in the last guy is remarkable.
»
» Is anyone trying this?

I tried the shampoo and found it too harsh, dried my hair way too much almost scoured the scalp, this is common to hear that. There is basically a formaldehyde type additive I believe that does this plus HCL, (hydrochloric acid also)

I instead used the 2 percent cream and applied it on my scalp every night and left it on. This is not adviseable. The ketoconazole is very strong and it is only recommended to be used several times per week, at most every other day.

After 4 months of Use my hair became much thinner almost see through on top front so i stopped using it. This may have been normal shock fallout or whatever they call it

After 6 to 12 months though, the hair all grew back and it was much healthier and much faster growing and much thicker than It was before I used the nizoral cream. In fact I have not used my pills in my regimen for about 18 months now, at all, just started back on them, and even though i stopped my regimen altogether the hair was still going great. This is extremely unusual, since usually if I stop my pill regimen within 2 months my hair looks like crap.

I recently started back on my pill regimen though. I stopped the nizoral cream back in 2008.

I want to start back on it since it did so well but I am not sure how much to use or how often. I am a bit hesitant since I had such a big shed, it kind of freaked me out though, but it did grow back great after that

I will say however, on my bald spot i had zero results. In my "thinning " areas I had great results.


#5

» I tried the shampoo and found it too harsh, dried my hair way too much
» almost scoured the scalp, this is common to hear that. There is basically a
» formaldehyde type additive I believe that does this plus HCL, (hydrochloric
» acid also)
»

Perrigo has a shampoo with ketoconazole 2% and is supposed to be much milder, cannot get it where i live but you might be luckier.


#6

Isn’t this kinda old news?

I’ve used Nizoral for years and it works great. Of course it’s much better for maintenance than actual regrowth (the results are nowhere near as dramatic as those photos), but it’s definitely effective.


#7

» Isn’t this kinda old news?
»
» I’ve used Nizoral for years and it works great. Of course it’s much better
» for maintenance than actual regrowth (the results are nowhere near as
» dramatic as those photos), but it’s definitely effective.

I would say it depends on the person and their age also and their level of baldness
older balder guys, who have been thinning much longer get less results

the younger you are and/or the hair that you are trying to get results in is just thinning instead of bald area…the better your results, according IMO to the study results posted

notice the older participants are the ones who got no results or less results

the younger guys are the ones with the more dramatic/positive results

course to me this is normal , bald areas are IMO almost impossible to regenerate after the follicles miniaturize and die

thinning areas, however are much easier to get results in, be it maintenance or regrowth

far as this being old news
to some board members its news…it certainly is better results than all the sham, pie in the sky HM “studies” people waste time talking about


#8

these photos are “semi professional” :wink:

no serious study offers unprofessional pics like those pics.:frowning:


#9

» these photos are “semi professional” :wink:
»
» no serious study offers unprofessional pics like those pics.:frowning:

the photos? thats called grasping at straws


#10

» » these photos are “semi professional” :wink:
» »
» » no serious study offers unprofessional pics like those pics.:frowning:
»
» the photos? thats called grasping at straws

Would you guys recommend trying the Nizoral shampoo during the shower or the lotion to apply after shampooing with a regular brand as they used in the study… It seems as though these are best case scenarios but hey, it’s worth a try, I could use more regrowth with my propecia. What do you guys think is the best method?