J Tissue Eng Regen Med. 2015 Jun 29. doi: 10.1002/term.2046. [Epub ahead of print]Wnt1a maintains characteristics of dermal papilla cells that induce mouse hair regeneration in a 3D preculture system.
Dong L1, Hao H1, Liu J1, Tong C1, Ti D1, Chen D1, Chen L1, Li M2, Liu H1, Fu X1, Han W1.
Hair follicle morphogenesis and regeneration depend on intensive but well-orchestrated interactions between epithelial and mesenchymal components. Therefore, an alternative strategy to reproduce the process of epithelial-mesenchymal interaction in vitro could use a 3D system containing appropriate cell populations. The 3D air-liquid culture system for reproducibly generating hair follicles from dissociated epithelial and dermal papilla (DP) cells combined with a collagen-chitosan scaffold is described in this study. Wnt-CM was prepared from the supernatant of Wnt1a-expressing bone marrow mesenchymal stem cells (BM-MSCs) that maintain the hair-inducing gene expression of DP cells. The collagen-chitosan scaffold cells (CCS cells) were constructed using a two-step method by inoculating the Wnt-CM-treated DP cells and epidermal (EP) cells into the CCS. The cells in the air-liquid culture formed dermal condensates and a proliferative cell layer in vitro. The CCS cells were able to induce hairregeneration in nude mice. The results demonstrate that Wnt-CM can maintain the hair induction ability of DP cells in expansion cultures, and this approach can be used for large-scale preparation of CCS cells in vitro to treat hair loss. Copyright © 2015 John Wiley & Sons, Ltd.
Copyright © 2015 John Wiley & Sons, Ltd.
Wingless-type mouse mammary tumour virus integration site; bone marrow mesenchymal stem cell; conditioned medium; dermal papilla; hairfollicles; regeneration; three-dimensional; tissue engineering