EXAMPLE 7
EDIHN-INDUCES NEW HAIR FOLLICLES IN HUMAN SKIN
MATERIALS AND EXPERIMENTAL METHODS
Grafting
[000210] Discarded human, adult scalp from the preauricular area obtained from plastic surgery was grafted onto immunodeficient (scid) mice. The graft was bandaged and allowed to heal, then was used in the wound healing study 3 months after grafting.
RESULTS
[000211 ] To determine whether human skin responded to EDIHN as did mouse skin, human skin was grafted onto SCID (immuno-deficient) mice and subjected to depilation by plucking and wound induction three days later. Seven days following wound induction, formation of new HF was observed in the human skin (Figure 2 IA; arrows indicate new HF) by hematoxylin and eosin staining of paraffin embedded tissue sections.
[000212]In additional experiments, adult human skin was grafted onto mice., abraded, and examined at 7 days post-abrasion. New HF were generated in the human skkx, which mimicked normal hair follicle formation during fetal development, as evidenced by staining for SlOO A6 or S100A4 (Figure 21B). P-7628-PC
[000213] The results of this Example show that EDIHN can be used to generate hair growth inhuman skin as for mouse skin.
EXAMPLE 8
It DOES NOT take 10 days post dermabrasion for follicles to begin forming in human skin. Just because follicles “are forming” however DOES NOT mean you will be able to see them. These are hair germs. Seeing the new hair will probably take a couple of months post procedure. Without the addition of wnt7a, not that many new follicles were made in the experiments. 140 hairs were made with wnt7a, vs. about 9 measley hairs without it. The epidermal growth factor receptor blocking drug is probably the second most important thing in the patent, and depilation three days before wounding was the third. All the other enhancements like arginine, beta catenin are less important than these.
Important: HERE IS WHAT I REALLY THINK--------------------------Follica’s procedure might produce pretty good hairs back in the wreath area of your head, where the scalp produced your “donor” hair. Unless they find a way to supress some autologus genes in the frontal scalp during this time with whatever exotic subtances, hairs made up front (if they can be made in the thinner, missing-water-layered-less-fatty-acids-in-the-skin FRONTAL SCALP) might be weaker hairs or even vellus-type hairs. OF COURSE I HOPE THAT ISNT THE CASE, and that the hairs made up front are big-thick and healthy and being on finasteride during their development (finasteride is mentioned in the patent along with some other anti-androgens) they will be resistant to DHT, but that may not be the case.
IF FOLLICA can merely work in your donor area, it will be able to “make” more hair for transplantion available to be moved up front, and in-effect “hair multiplication”. If we could simply “come up with” 10,000 more hairs that could be moved up front, most of us would be very well-off with just a regular transplant. Its my hope that one will be able to shave their donor area-----have follica performed------and have very thick donor hair in the wreath grow in so and FUE surgeon can move it up front without thinning out the back of your head. I’d love for them to hit a home run, but would happily embrace a -smaller-success in the wreath.
For any of you who may be newbies wondering about baldness-------------you inherit a variant of the androgen receptor gene that can express itself more or less strongly depending on genetic chance. If it expresses itself strongly with CAG-repeates, the hair outside your wreaths androgen receptor’s are much more adept at uptaking male hormone, especially dihydrotestosterone. Having alot of DHT in the scalp tissue (bald men have been shown to have more DHT in their scalps, but not in the blood, indicating that the alpha five reductase enzymes in the root sheaths of each and ever hair follicle on your head are probably more active than a guy with all his hair) has been shown to be able to invigorate androgen receptors even MORE. One too much androgen is uptaken…eventually something happens to the hair follicle and a different set of genetic instructions are obeyed within it. The dermal papilla starts cranking out way too much DKK_1, which leads to the cell death of the keratincoyetes in the root sheath…these dead cells in turn hanging around the infidulum is probably why the immune system begins attacking the hair follicle and TGF beta and excessive collagenous deposition is downstream from it. That is probably “baldness”, as the immune system is going to try to eliminate dead cells and anything around the area is going to be effected by all the infllammatory subtstances the immune system will use. Baldness looks like organ rejection microscopically. Its been shown that wreath area hairs can succumb to testosterone and DHT if experiments give enough of them to the hairs…so I dont believe their is some magic difference in the hair’s basic characteristics but a difference in the amount of DHT the hair can make via its alpha five reductase enzyme in their root sheaths and a big difference in how well the hairs androgen receptors work and how chemcially stable they are. In a way male baldness is probably because you have too-well-working-androgen-receptors and too-well-working-alpha-five-reductase-enzymes in your hair. You are “too manly” in a place you dont want to be.
