Dear Friends/Critics,
Hair Doubling of 6 single hair follicle into 12 new single hair follicle at the recipient (1 recipient site showing 2 follicles in a single FU). This is the first case study, 9 more case studies will be conducted for the coming 2 months. This will be an important breakthrough as there are approximately 1300 dermal papilla cells and additional dermal sheath cells per follicle which may allow us hair doubling in multiples with only few follicular units. In both bisected follicles, we have also injected progenitor cd200, cd34, ck19, versican and alkaline phosphate positive, epidermal and dermal stem cell respectively. I will explore the legality aspect of this technique in the western world as this technique can be replicated partially even without isolating and activating stem cells. I wish to patent this technique as proto hair organoids and will offer the process training to the fellow doctors with the license fee and continuous up-gradation on the process. With either German OR Japanese team shortly, Dr.Nigam’s brand would have better credibility value as experienced and senior bio-technologists and tissue engineers become a part of the brand with their rapport in published scientific world and state-of-art labs. As you are aware of Dr.Nigam’s brand stands for applied research and state-of-art, chain of hair transplant clinics which offers access to clinical new procedural development as unpublished data before final procedures are selected and starts undergoing specific clinical trials.
please explain what we are seeing here? are these implanted grafts at the recipient site? are these regrowing grafts in the donor site? There is really not much one can make of these images without an explanation.
Neversay,
Yes we will have to apply the tattoo mark again on the fading tatoo mark as tattoo scab came out when the study participant washed his hair.
I chose single hair follicle from the nape of the neck for the clarity in documentation ,as i can shave anytime and more the simplified the study, better clarity in counting etc.I remember in vitro bisection was always at the back of your and my mind.I am eagerly waiting to add inducible DP/DC cell culture in the next study for multiple growth of HF from few HF’S.Lauster team specializes in Inducible DP/DC culture.
» Thank you for the effort.
»
» Why did you choose to use single hair follicles and not doubles?
»
» it seems like you might have to re-apply the tattoo markers. When will the
» next update be?
»
» Its exciting. If you succeed in this, and share your results properly with
» the world, it should change the hair transplant industry for good.
»
» Good luck, and grow babies grow!
Hairmann,
These are 12 bisected(in vitro) single hair follicles from the nape of the neck implanted to the left recipient temple of the patient in this study.
Although 3 bisected follicle’s had propped out and discarded while injecting activated stemcell solution,still we can see 13 single hair follicles from 12(3 discarded bisected follicles, hence 13 new follicles from 9 bisected follicles) bisected single follicles at the recipient on day 7 itself.
No these are not regrowing follicles on the donor site ,that will be the part of subsequent studies.
Next study will add cultured DP/DC cells to the bisected follicle,we are hoping more than doubling of HF’s in numbers in the next study.
These cases will be followed up for rate of growth ,follicle diameter ,angle of growth etc.
Both the bisected parts have dp cells and epithelial stem cells.The line of bisection in vitro under 50x magnification was at DP level near the line of auber.
Few grafts were extracted from donor separately to isolate and activate the progenitor stemcells which were subsequently injected into both the bisected follicles.
» dear dr nigam,
»
» please explain what we are seeing here? are these implanted grafts at the
» recipient site? are these regrowing grafts in the donor site? There is
» really not much one can make of these images without an explanation.
»
» »
» »
» »
» »
» »
» »
» »
» » For more information visit www.drnigams.net
YOu say both parts contain DP cells? Does that mean you bisect the follicle through the middle? Gho takes just the upper half (roughly).
I was not interested in ‘doubling’ transplants, but if it was available at a good price, i would be cured! I would say im 3000 follicles away from being VERY happy with my hair.
My only advice would be to keep it simple. If you can achieve doubling that produces full terminal hairs with a high yield, dont use HM or anything else.
The market for this would be huge. Looking forward to your updates.
» Another question:
»
» Lets assume you get great results from these case studies (90% yield of
» terminal hairs)…
»
» You are doing case studies with a very low number of grafts. How would you
» step it up to large amounts of grafts? 2000, 3000, 4000?
»
» Would the higher extraction rate lead to complications do you think?
Right. We have no case complete with FUE or FUT of Nigam, we don’t know how he operates…
No,bisect at the level of dermal papilla,just below the line ofauber.THE upper part has both ,bulge epithelial stem cells and little DP cells.
The lower bisected part has DP/DC cells plus injected epithelial cd200,cd34,k19 of the mid follicle bulge.
I know the market is big for doubling as it will have higher success rate ,but the ultimate goal is to improve HM, when we consult at our clinic we take guarantee of NW7 to NW3 by using combination technique.
Yes you are correct less technical people will get confused…i know this even when i am typing…
» You say both parts contain DP cells? Does that mean you bisect the follicle
» through the middle? Gho takes just the upper half (roughly).
»
» I was not interested in ‘doubling’ transplants, but if it was available at
» a good price, i would be cured! I would say im 3000 follicles away from
» being VERY happy with my hair.
»
» My only advice would be to keep it simple. If you can achieve doubling that
» produces full terminal hairs with a high yield, dont use HM or anything
» else.
»
» The market for this would be huge. Looking forward to your updates.
Neversay,
Which complications are you expecting from in vitro bisection apart from limitations of extraction from safe donor area of a 3 step FUE.
In vivo Longitudnal bisection of graft blindly which we are not into will have unsuitable or transected FU possibility.
You can put forward other possible complications.
And focus on HM has to be maintained as ,an improved combination of HD with HM and DP cell culture should be able to give best possible result as on 2013.
» Another question:
»
» Lets assume you get great results from these case studies (90% yield of
» terminal hairs)…
»
» You are doing case studies with a very low number of grafts. How would you
» step it up to large amounts of grafts? 2000, 3000, 4000?
»
» Would the higher extraction rate lead to complications do you think?
» Neversay,
» Which complications are you expecting from in vitro bisection apart from
» limitations of extraction from safe donor area of a 3 step FUE.
» In vivo Longitudnal bisection of graft blindly which we are not into will
» have unsuitable or transected FU possibility.
» You can put forward other possible complications.
» And focus on HM has to be maintained as ,an improved combination of HD with
» HM and DP cell culture should be able to give best possible result as on
» 2013.
»
» » Another question:
» »
» » Lets assume you get great results from these case studies (90% yield of
» » terminal hairs)…
» »
» » You are doing case studies with a very low number of grafts. How would
» you
» » step it up to large amounts of grafts? 2000, 3000, 4000?
» »
» » Would the higher extraction rate lead to complications do you think?
Complications…It has been claimed in the past that donor regeneration can be effected by number of grafts extracted. If too much extracting is done to the donor, the tissue needs alot of repair, and thus it might effect the regeneration %.
In theory higher graft numbers can be used, provided the donor region is nice and healthy (full of hair!). What is the maximum number of grafts you have extracted for doubling so far? I have become more excited about doubling than HM purely because right now doubling could provide garenteed result. Will you be providing case studies that use a higher number of follicles? maybe 500? 1000?
I had 1200 grafts with Dr gho but it left me wondering why noone has tried what you are trying now! Extract, bisect, implant.
My work plans have changed, and im now being sent to Mumbai in Late march.
The quality of the resulting hairs must be measured or else doubling means nothing. Making a single hair into two half-size hairs does not give more total coverage.
Cal,this is not simple transverse bisection and implantation but the bisection at a level of follicle that DP cells are present in both the bisected follicle.
To these bisected follicles are added epithelial and mesenchymal progenitor cells extracted from few grafts from the patients scalp ( can also be extracted from the body).
We will follow the diameter and character of hair regrowth,day 10 results are encouraging even regarding the diameter of the new growing follicles.
Another advantage with doubling is you get instant growth of follicles as against fue/fut wherein the follicle undergoes dormancy before it slowly starts growing over a period of months.
Just wait for few more case studies…this would be a much improved and aggressive Hair doubling not just donor doubling over a period of years that my friend dr gho’s Blind longitudinal bisection technique offers.
We are not going to stop at that ,To the bisected progenitor follicle bulge stemcellls,ors stemcells,dermal cup sheath cells are already added ,with addition of DP culture cells,we are expecting more than 1 follicle per bisected follicle.
» » Neversay,
» » Which complications are you expecting from in vitro bisection apart from
» » limitations of extraction from safe donor area of a 3 step FUE.
» » In vivo Longitudnal bisection of graft blindly which we are not into
» will
» » have unsuitable or transected FU possibility.
» » You can put forward other possible complications.
» » And focus on HM has to be maintained as ,an improved combination of HD
» with
» » HM and DP cell culture should be able to give best possible result as on
» » 2013.
» »
» » » Another question:
» » »
» » » Lets assume you get great results from these case studies (90% yield
» of
» » » terminal hairs)…
» » »
Neversay
Is longitudinal bisection with an fue punch (even if it is modified as triple wave punch)anything different from planned partial FUE.
Isn’t the complication you are mentioning, a normal complication of a mega FUE with close FU extraction.Is it not that all the surgeons keep a gap between two sessions of any HT.
Is it not that 3000 FUE from safe donor area are common across the clinics without limiting complication.
These partial longitudinal bisections of our dr friend(which is claimed as hair stemcell transplant? without even isolating any stemcell not to mention the subsequent activation to progenitor stemcells) will also give rise to donor doubling,but since it is a blind technique it will have more unsuitable grafts at the recipient ,donor will always grow back because it will always have some part of DP as it is embedded in the subcut tissue.
Since my doubled hair are all implanted at the recipient why will my extractions heal differently from normal fue with 0.6mm punch(with ECM).
Yes if the patient wants to re implant certain number of bisected FU at the donor i may reduce extraction by at least 30% in one session before the donor heals and is vascularized.
Will start the case study on a patient from 1st feb with 3000 extractions in 3 to 4 days and double the FU’S to 6000 or more within 15 days with visible new growth of hair.My challenge is to prove HAIR DOUBLING will be preferred choice(as the HM evolves) in the years to come than fue or HSC(only problem which many surgeons will face is that it is time consuming and trained manpower dependent ,thats where east will be able to offer more doublings at one fifth the price due to available of large teams performing doubling).
As grafts in our HAIR DOUBLING
1)can be more than doubled,
2)safe dissection under magnification,
3)improving quality and number of follicles with activated stemcells and DP culture cells
4) and instant growth of dissociated follicle as against slow growth of new Follicle over a period of months with traditional FUE.
I hope with the subsequent case studies we all would be able to convince ourselves more on this HD technique.
Remember i am debating techniques and claim of doubling and not competency of my doctor friend(i understand his hands are tied with the european regulations which are even tough than american FDA regulation hence he has to limit himself to dipping the grafts in the preservation medium, which is nothing more than graft preservation and little nutrition ).
It’s good you are coming in march as DP cell culture would have been added to both our HD and HM by than and you would have watched at least one case of large doubling on the forum.
» » » step it up to large amounts of grafts? 2000, 3000, 4000?
» » »
» » » Would the higher extraction rate lead to complications do you think?
»
» Complications…It has been claimed in the past that donor regeneration
» can be effected by number of grafts extracted. If too much extracting is
» done to the donor, the tissue needs alot of repair, and thus it might
» effect the regeneration %.
»
» In theory higher graft numbers can be used, provided the donor region is
» nice and healthy (full of hair!). What is the maximum number of grafts you
» have extracted for doubling so far? I have become more excited about
» doubling than HM purely because right now doubling could provide garenteed
» result. Will you be providing case studies that use a higher number of
» follicles? maybe 500? 1000?
»
» I had 1200 grafts with Dr gho but it left me wondering why noone has tried
» what you are trying now! Extract, bisect, implant.
»
» My work plans have changed, and im now being sent to Mumbai in Late march.
Dr Nigam—The healing in the recipient site is very good----the best I have ever seen for 10 days. The activated stem cell solution must help the healing. Do you think this will help reduce the redness in the recipient area that is visible after a regular transplant.
Now that you have the activated stemcell solution could you try implanting plucked follicles. With the new solutions and inducible DP/DC cell culture you have the ingredients to help the plucked hair follicle to grow. One of your techs could pluck the follicle and hand it to the second tech who would insert it into the recipient sites and add the activated stemcell solution. You could do between 3000 to 4000 follicles a day with no bisecting.
I was also thinking you could try plucking follicles that are a inch long and placing them in the recipient sites. If they kept growing like the bisected follicles in your first trial you could go to your clinic and come out with a full head of hair.
» Hairmann,
» These are 12 bisected(in vitro) single hair follicles from the nape of the
» neck implanted to the left recipient temple of the patient in this study.
» Although 3 bisected follicle’s had propped out and discarded while
» injecting activated stemcell solution,still we can see 13 single hair
» follicles from 12(3 discarded bisected follicles, hence 13 new follicles
» from 9 bisected follicles) bisected single follicles at the recipient on
» day 7 itself.
maybe it’s just me but I am not understanding your calculation.
if you bisect 6 follicles then you get 12 bisected follicles of which 3 propped out and were discared leaving us with 9 bisected follicles. How do you come to the figure of 13 new single hair follicles from 12?
Mell your suggestion reminds me of what JAHODA the famous scientist from uk did when he actually transplanted a male hair follicle after washing off any blood and dermal tissue, on a female fore arm and the follicle grew back(he has published this paper) as dp is immune genetically safe,but the scientists are going slow on transgender HT due to Allogenic tough regulations.
Yes addition of progenitor stemcells, PRP, ECM DOES HELP HEALING of both recipient and donor.
I will experiment as you said although my major worry with your suggestion is the thickness of the new hair.Will do this in mid feb as i get better prepared with my inducible DP culture .
» Dr Nigam—The healing in the recipient site is very good----the best I
» have ever seen for 10 days. The activated stem cell solution must help the
» healing. Do you think this will help reduce the redness in the recipient
» area that is visible after a regular transplant.
»
»
» Now that you have the activated stemcell solution could you try implanting
» plucked follicles. With the new solutions and inducible DP/DC cell culture
» you have the ingredients to help the plucked hair follicle to grow. One
» of your techs could pluck the follicle and hand it to the second tech who
» would insert it into the recipient sites and add the activated stemcell
» solution. You could do between 3000 to 4000 follicles a day with no
» bisecting.
»
»
» I was also thinking you could try plucking follicles that are a inch long
» and placing them in the recipient sites. If they kept growing like the
» bisected follicles in your first trial you could go to your clinic and come
» out with a full head of hair.
»
»
» Thanks for your time Mell
» Cal,this is not simple transverse bisection and implantation but the
» bisection at a level of follicle that DP cells are present in both the
» bisected follicle.
» To these bisected follicles are added epithelial and mesenchymal progenitor
» cells extracted from few grafts from the patients scalp ( can also be
» extracted from the body).
» We will follow the diameter and character of hair regrowth,day 10 results
» are encouraging even regarding the diameter of the new growing follicles.
» Another advantage with doubling is you get instant growth of follicles as
against fue/fut wherein the follicle undergoes dormancy before it slowly
starts growing over a period of months.
» Just wait for few more case studies…this would be a much improved and
» aggressive Hair doubling not just donor doubling over a period of years
» that my friend dr gho’s Blind longitudinal bisection technique offers.
»
» We are not going to stop at that ,To the bisected progenitor follicle
» bulge stemcellls,ors stemcells,dermal cup sheath cells are already added
» ,with addition of DP culture cells,we are expecting more than 1 follicle
» per bisected follicle.
Nigam the problem today is that, despite propecia, the baldness does not stop, so for the average person 10.000 -12.000 grafts need to have for a good situation.
10000 * 3.5 $ for one graft (your cost) = $ 35,000 + flight + hotel + time
You can have a relook at the pics on my site for enlarged view, one of the bisected follicle is showing new growth of two follicles and all the 12 implanted sites are also showing new hair growth(remember activated stem cells were also injected on all recipient sites into bisected follicles)…hence 13(L1 to L12 ) from 9 bisected follicular parts (not 9 follicles).
» » Hairmann,
» » These are 12 bisected(in vitro) single hair follicles from the nape of
» the
» » neck implanted to the left recipient temple of the patient in this
» study.
» » Although 3 bisected follicle’s had propped out and discarded while
» » injecting activated stemcell solution,still we can see 13 single hair
» » follicles from 12(3 discarded bisected follicles, hence 13 new follicles
» » from 9 bisected follicles) bisected single follicles at the recipient on
» » day 7 itself.
»
» maybe it’s just me but I am not understanding your calculation.
»
» if you bisect 6 follicles then you get 12 bisected follicles of which 3
» propped out and were discared leaving us with 9 bisected follicles. How do
» you come to the figure of 13 new single hair follicles from 12?
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