Substantial sex-dependent differences in the response of human scalp hair follicles to estrogen stimulation in vitro advocate gender-tailored management of female versus male pattern balding.
Department of Dermatology, University Hospital Hamburg-Eppendorf, University of Hamburg, Hamburg, Germany.
In this study, it was investigated how estrogens (17-beta-estradiol, E2) affect the estrogen receptor (ER) expression and gene regulation of male versus female human scalp hair follicles in vitro. Anagen VI follicles from frontotemporal scalp skin were microdissected and organ-cultured for up to 9 d in the presence of E2 (1-100 nm). Immunohistochemistry was performed for ERbeta-expression, known to be predominant in human scalp hair follicles, and for TGF-beta2-expression (as negative key hair growth modulator), and E2-responsive genes in organ-cultured human scalp hair follicles (48 h, 10 nM) were explored by cDNA microarray, using a commercial skin focus chip (Memorec, Cologne, Germany). The distribution pattern of ERbeta and TGF-beta2-immunoreactivity differed between male and female hair follicles after 48 h culture. Of 1300 genes tested, several genes were regulated sex-dependent differently. The study reveals substantial sex-dependent differences in the response of frontotemporal human scalp hair follicles to E2. Recognition and systematic dissection of the E2-dependent gene regulation will be crucial for the development of more effective, gender-tailored management strategies for female versus male pattern balding.
» Substantial sex-dependent differences in the response of human scalp
» hair follicles to estrogen stimulation in vitro advocate gender-tailored
» management of female versus male pattern balding.
»
» Department of Dermatology, University Hospital Hamburg-Eppendorf,
» University of Hamburg, Hamburg, Germany.
»
» In this study, it was investigated how estrogens (17-beta-estradiol, E2)
» affect the estrogen receptor (ER) expression and gene regulation of male
» versus female human scalp hair follicles in vitro. Anagen VI follicles
» from frontotemporal scalp skin were microdissected and organ-cultured for
» up to 9 d in the presence of E2 (1-100 nm). Immunohistochemistry was
» performed for ERbeta-expression, known to be predominant in human scalp
» hair follicles, and for TGF-beta2-expression (as negative key hair growth
» modulator), and E2-responsive genes in organ-cultured human scalp hair
» follicles (48 h, 10 nM) were explored by cDNA microarray, using a
» commercial skin focus chip (Memorec, Cologne, Germany). The distribution
» pattern of ERbeta and TGF-beta2-immunoreactivity differed between male and
» female hair follicles after 48 h culture. Of 1300 genes tested, several
» genes were regulated sex-dependent differently. The study reveals
» substantial sex-dependent differences in the response of frontotemporal
» human scalp hair follicles to E2. Recognition and systematic dissection of
» the E2-dependent gene regulation will be crucial for the development of
» more effective, gender-tailored management strategies for female versus
» male pattern balding.
»
» PMID: 16382674 [PubMed - indexed for MEDLINE]
» Substantial sex-dependent differences in the response of human scalp
» hair follicles to estrogen stimulation in vitro advocate gender-tailored
» management of female versus male pattern balding.
»
» Department of Dermatology, University Hospital Hamburg-Eppendorf,
» University of Hamburg, Hamburg, Germany.
»
» In this study, it was investigated how estrogens (17-beta-estradiol, E2)
» affect the estrogen receptor (ER) expression and gene regulation of male
» versus female human scalp hair follicles in vitro. Anagen VI follicles
» from frontotemporal scalp skin were microdissected and organ-cultured for
» up to 9 d in the presence of E2 (1-100 nm). Immunohistochemistry was
» performed for ERbeta-expression, known to be predominant in human scalp
» hair follicles, and for TGF-beta2-expression (as negative key hair growth
» modulator), and E2-responsive genes in organ-cultured human scalp hair
» follicles (48 h, 10 nM) were explored by cDNA microarray, using a
» commercial skin focus chip (Memorec, Cologne, Germany). The distribution
» pattern of ERbeta and TGF-beta2-immunoreactivity differed between male and
» female hair follicles after 48 h culture. Of 1300 genes tested, several
» genes were regulated sex-dependent differently. The study reveals
» substantial sex-dependent differences in the response of frontotemporal
» human scalp hair follicles to E2. Recognition and systematic dissection of
» the E2-dependent gene regulation will be crucial for the development of
» more effective, gender-tailored management strategies for female versus
» male pattern balding.
A key finding of this study was that in men, the application of estrogen led to a dose-dependent REDUCTION in TGFb-2, which is known to be a key suppressor of hair follicle growth in androgenetic alopecia. That shows a clearly beneficial effect of estrogen in males.
This is an advertising site for paid
advertisers to showcase successful hair restoration results only. It is not the
mandate of this site to engage in the discussion of failed, unsuccessful
procedures, lawsuits, litigations, refunds or complaint cases. Surgical hair
restoration procedures carry risks. Please do thorough research, consult your
own physician and investigate a doctor's background carefully before making a
decision. By proceeding to use our site, you agree to abide by our Terms of Use & Privacy Policy at http://hairsite.com/terms-of-use/ where you can also find a list of HairSite's sponsoring physicians.