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Patent


#1

http://www.freepatentsonline.com/y2010/0034856.html

Inventors:
Gho, Conradus Ghosal (Bunde, NL)

Claims:

  1. A method for the reproduction of hair, which method comprises the following steps: (a) removing hair in the anagen phase from one or more donor areas in such a way that the hair stem cells which are responsible for hair growth are still attached to the hair removed; and (b) bringing the hair stem cells of the hair removed into contact with a medium which contains extracellular matrix components or substitutes there; and © implanting the hair of step b) in the scalp.

  2. The method according to claim 1, in which the medium which contains extracellular matrix components or substitutes therefore has a viscous consistency.

  3. The method according to claim 1, in which hair in the anagen phase is removed by plucking the hair from one or more donor areas, followed by selection of suitable hairs in the anagen phase.

  4. An application of extracellular matrix components or substitutes therefore for the reproduction of hair.

Description:

The essential growth structures of hair are the so-called hair follicles which are present in the skin. These hair follicles produce hair follicle cells or keratinocytes. During their journey to the surface of the skin the cytoplasm of these cells is converted by a large number of complex processes into the tough and elastic material which is known as hair. The growth cycle of hair can be subdivided into three phases: the anagen phase (‘growth phase’), the catagen phase (‘transitional phase’) and the telogen phase (‘death phase’). The hair follicle is unique in the cyclic nature of hair formation and hair growth. It is the only part of the body that has a growth nucleus, from which new hairs can be produced after removal of the old hair.

Human beings usually find baldness undesirable from a cosmetic and aesthetic point of view. Baldness occurs frequently, however, and it is a known phenomenon that men in particular become balder as they get older. It does also occur in women, however, and is then in particular highly undesirable from a cosmetic and aesthetic point of view.

A known technique for combating baldness is the transplantation of hair. With this procedure hair, including the skin, is removed from a donor area covered with hair which is often located on the back of the head, and is cut into small pieces which usually only contain one to three hairs. These pieces are then implanted in the bald area (receptor area). A significant disadvantage of this technique is that this is at the expense of the donor area. After all, hair is removed from this area and this hair does not grow back. This transplantation technique therefore offers limited possibilities.

It is known that hair follicle cells can be cultured from plucked human hair. It is also known that it is difficult to form a differentiated epidermis or a completely developed epidermis with the aid of such cultured cells, both in vitro and in vivo. Cultured hair follicle cells from mice can stimulate hair growth when they are implanted in test animals.

A method for the reproduction of hair in human beings is described in European patent application 0 236 014, in which epidermal follicle cells of the desired hair type are removed from the head hair of a patient. The epidermal follicle cells are then cultured in a culture medium which preferably contains growth factors. An opening is then made in the epidermis of the patient’s scalp and the cultured epidermal follicle cells are introduced via this opening into the dermis next to the epidermis. The disadvantage of this method is that it includes an invasive method and that the cells are not placed in a targeted manner, so that many cells are needed and the probability of the regeneration of hair is less.

Another method for the reproduction of hair is described in European patent application 0 971 679. Here the hair is removed from a donor area in such a way that new hairs come back in place thereof, while new hair follicle cells are cultured from the hairs removed, from which cells can once again form new hair. Renewed hair growth can thus be achieved without this being at the expense of the donor area. To this end hair follicle cells removed in the anagen phase are cultured for 1 hour to 40 days in a serum-free keratinocyte culture medium. A disadvantage of this method is the long time that is needed for culturing the hair follicle cells.

It is the object of this invention to overcome one or more of the above-mentioned problems, and thus to provide a method for the reproduction of human hair in which a long culturing time is not required. Extracellular matrix is produced by the (support) cells around the hair follicle. From our own research it is apparent that this Extracellular Matrix is essential for hair growth. In this invention the hairs are no longer cultured in a keratinocyte culture medium but are simply immersed for a short time in a medium which contains extracellular matrix or substitutes therefor. The hair is then put back. With this technique several hairs can grow as a result of putting back a single hair. The method according to the invention is moreover patient-friendly, because only plucked hairs can be used and no invasive method or anaesthesia is necessary, the patient does not have to come back for a second treatment after culturing in order to have the hairs implanted after culturing. A further advantage is that the donor area is left intact.

This invention thus relates to a method for the reproduction of hair, which method comprises the following steps:

*
      o (a) removing hair in the anagen phase from one or more donor areas in such a way that the hair stem cells which are responsible for hair growth are still attached to the hair removed; and
      o (b) bringing the hair removed into contact with a medium which contains extracellular matrix components or substitutes therefor; and
      o (c)implanting the hair of step b) in the scalp.

According to this invention it is thus not necessary to culture the hair follicle cells; merely immersing the hair in the medium which contains extracellular matrix components or substitutes therefor is sufficient. The hair is then put back in the scalp, after which several hairs grow out of this one hair.

In step a) of the method according to this invention hair in the anagen phase is removed from one or more donor areas in such a way that the hair stem cells which are responsible for hair growth are still attached to the hair removed.

As has already been stated above, hair growth comprises three phases: an anagen, catagen and telogen phase. Only hairs which are in the anagen phase are suitable for the method according to the invention. Compared with hairs in the catagen and telogen phase such hairs in the anagen phase are characterized in that they have a bulbus—often pigmented—with a shape that is characteristic of hairs in the anagen phase, on the underside of the hair. This is generally known and a hair in the anagen phase is therefore immediately recognizable for an experienced eye by the shape of the bulbus. Use of a microscope can give a definitive answer in cases of doubt. The removal of hair in the anagen phase can be effected in various ways, provided that the bulbus which is characteristic of hair in the anagen phase is still attached to the hair removed.

Since the difference between hairs in the different growth phases is very clearly visible when the hairs have been removed, the hairs are for example removed from the donor area by plucking hair from the donor area and selecting the hairs plucked in the anagen phase. Tweezers are for example highly suitable for plucking hair from the donor area. The hair can also be plucked from the scalp by hand, however.

In step b) of the method according to the invention the hair removed is brought into contact with a medium which contains extracellular matrix components or substitutes therefor.

To this end a plucked hair can for example be immersed for a short time, e.g. 2 seconds, in the medium which contains extracellular matrix components or substitutes therefor. A longer contact time between the hair removed and the medium which contains extracellular matrix components or substitutes therefor is of course also possible but is not required. The culturing step which is described in European patent application 0 971 679 is thus not needed.

The term “extracellular matrix components” is a term known to all in the art. Substitutes therefor are also known in the art. Examples of Extracellular Matrix components are collagen, laminin, etc. These components are already available for cell culture. Examples thereof are Collagen I to IV inclusive, from Sigma/Aldrich, or Alminin, from Merck.

It is not necessary for the whole hair to be immersed in the medium which contains extracellular matrix components or substitutes therefor. Immersing the part of the hair to which the stem cells are attached is sufficient for the reproduction of hair by the method according to this invention.

In the last step c) the hair of step b) is implanted in the scalp. This hair—or at least the part of the hair to which the stem cells are attached—has been in contact with a medium which contains extracellular matrix components or substitutes therefor, and probably as a consequence of this the hair stem cells which are located in that part have been ‘activated’, so that they too can develop into hairs. As a consequence of this the implantation of the single hair of step b) results in the growth of one or several hairs.

The medium which contains extracellular matrix components or substitutes therefor preferably has a viscous consistency (e.g. a hydrogel). As used here, the term “medium” refers to a substance which contains important nutritional components, such as growth factors and trace elements.

As has already been discussed above, it is preferable that hair in the anagen phase is removed by plucking the hair from one or more donor areas, followed by selection of suitable hairs in the anagen phase.

The invention furthermore relates to the application of extracellular matrix components or substitutes therefor for the reproduction of hair. Such an application of extracellular matrix components has not been described previously.


#2

» http://www.freepatentsonline.com/y2010/0034856.html

He he, clever guy, this man named Gho Ghosal Conradus …

Seems he is back on stage. But this time he has a real big weapon in his hands.


#3

» http://www.freepatentsonline.com/y2010/0034856.html

More Gho bullsht. How does it go again? “Fool me once, shame on you. Fool me twice, shame on me.”


#4

» » http://www.freepatentsonline.com/y2010/0034856.html
»
» More Gho bullsht. How does it go again? “Fool me once, shame on you.
» Fool me twice, shame on me.”

Heres a link to a journal and their website by them - better photos than they used to show,but I’m still not convinced

Oh sh*t Amsterdam would be very easy for me to get to as well - Last time I went there a good looking woman asked me do I have the time - I looked at my watch stupid me (Amsterdam is well known for prositutes) - The amount of beggers on the street,was a lot,to.It was as if they knew you was a tourist - Loads of McDonalds,to - horrible place

http://www.dewittevrouwenhof.nl/algemeen/layout_images/brochures/hsi_haarjournaal_08_uk.pdf

Heres their website

http://www.hasci.com/Default.aspx


#5

» » » http://www.freepatentsonline.com/y2010/0034856.html
» »
» » More Gho bullsht. How does it go again? “Fool me once, shame on you.
» » Fool me twice, shame on me.”
»
» Heres a link to a journal and their website by them - better photos than
» they used to show,but I’m still not convinced
»
» Oh sh*t Amsterdam would be very easy for me to get to as well - Last time
» I went there a good looking woman asked me do I have the time - I looked at
» my watch stupid me (Amsterdam is well known for prositutes) - The amount of
» beggers on the street,was a lot,to.It was as if they knew you was a tourist
» - Loads of McDonalds,to - horrible place
»
» http://www.dewittevrouwenhof.nl/algemeen/layout_images/brochures/hsi_haarjournaal_08_uk.pdf
»
» Heres their website
»
» http://www.hasci.com/Default.aspx

actually the results on that site look promising to me… and if i understand correctly, it seems they are offering the treatment already today?? They even already have pricing tables, however the treatment is far more expensive than normal transplant surgery. The site also only seems to be in dutch only… being german i was able to understand most of it, however a translation would be apreciated :slight_smile:
However from what i understand this is smoething like FUE except that the extracted follice is only partly extracted allowing the follicle to be cloned. I have my doubts that that acutally works effectively… I would like some clinical proof of that concept before i pay a sh*tton for treatment.


#6

» » » » http://www.freepatentsonline.com/y2010/0034856.html
» » »
» » » More Gho bullsht. How does it go again? “Fool me once, shame on you.
»
» » » Fool me twice, shame on me.”
» »
» » Heres a link to a journal and their website by them - better photos
» than
» » they used to show,but I’m still not convinced
» »
» » Oh sht Amsterdam would be very easy for me to get to as well - Last
» time
» » I went there a good looking woman asked me do I have the time - I looked
» at
» » my watch stupid me (Amsterdam is well known for prositutes) - The amount
» of
» » beggers on the street,was a lot,to.It was as if they knew you was a
» tourist
» » - Loads of McDonalds,to - horrible place
» »
» »
» http://www.dewittevrouwenhof.nl/algemeen/layout_images/brochures/hsi_haarjournaal_08_uk.pdf
» »
» » Heres their website
» »
» » http://www.hasci.com/Default.aspx
»
» actually the results on that site look promising to me… and if i
» understand correctly, it seems they are offering the treatment already
» today?? They even already have pricing tables, however the treatment is far
» more expensive than normal transplant surgery. The site also only seems to
» be in dutch only… being german i was able to understand most of it,
» however a translation would be apreciated :slight_smile:
» However from what i understand this is smoething like FUE except that the
» extracted follice is only partly extracted allowing the follicle to be
» cloned. I have my doubts that that acutally works effectively… I would
» like some clinical proof of that concept before i pay a sh
tton for
» treatment.


#7

» However from what i understand this is smoething like FUE except that the
» extracted follice is only partly extracted allowing the follicle to be
» cloned. I have my doubts that that acutally works effectively… I would
» like some clinical proof of that concept before i pay a sh*tton for
» treatment.

Hm, not sure, but regarding “clinical proof” it seems thers is already something “secret” (?) going on out there …
http://www.hasci.com/uploads/downloads/Rectificatie.dr.B.Feriduni.pdf

Some well-known Docs from Europe, like Dr. Feriduni or Dr. De Reys or Dr. Neidel, already signed this paper I found within the “NEUWS” section on the site.


#8

» » However from what i understand this is smoething like FUE except that
» the
» » extracted follice is only partly extracted allowing the follicle to be
» » cloned. I have my doubts that that acutally works effectively… I would
» » like some clinical proof of that concept before i pay a sh*tton
» for
» » treatment.
»
» Hm, not sure, but regarding “clinical proof” it seems thers is already
» something “secret” (?) going on out there …
» http://www.hasci.com/uploads/downloads/Rectificatie.dr.B.Feriduni.pdf
»
» Some well-known Docs from Europe, like Dr. Feriduni or Dr. De Reys or Dr.
» Neidel, already signed this paper I found within the “NEUWS” section on the
» site.

Wow, this is really starting to sound like they might be on to something… Any Dutch people care to translate the gist of it?


#9

Wow, this is really starting to sound like they might be on to something…nothing… this already exists to the many years, and nobody showed swims, no photo, no image, nothing, nothing, nothing, doctor GHO= NOTHING…


#10

» … this already exists to the many
» years, and nobody showed swims, no photo, no image, nothing, nothing,
» nothing, doctor GHO= NOTHING

Why “nothing”? What do you expect?

Go to the “NEUWS” section on this site:
http://www.hasci.com/default.aspx

There you will find a VIDEO about this process & technique, patented and practiced by Dr. Gho.
Furthermore, look at the first (start) post above. There (patent) you can READ in English, HOW and WHY his technique works.

It seems, some antagonistic Docs out there, already did a big mistake against Gho, so they have been forced - indirect - to “confirm” Gho’s successful technique, because it definitely WORKS and that has been proven already!

He he, it seems they all (Docs) have just disgraced themselves with their attacks (and penitently signatures after that) against Dr. Gho … :smiley:

I would love to see their faces during their signature act. :smiley:

By the way - Here you can READ (translate) what happened:


#11

Gho was on TV here last week and said there will be a study/scientific article published in a medical/science magazine. BTW, all the McDonalds in Amsterdam is thanks to the USA,I believe.

» » … this already exists to the many
» » years, and nobody showed swims, no photo, no image, nothing, nothing,
» » nothing, doctor GHO= NOTHING
»
» Why “nothing”? What do you expect?
»
» Go to the “NEUWS” section on this site:
» http://www.hasci.com/default.aspx
»
» There you will find a VIDEO about this process & technique,
» patented and practiced by Dr. Gho.
» Furthermore, look at the first (start) post above. There (patent) you can
» READ in English, HOW and WHY his technique works.
»
» It seems, some antagonistic Docs out there, already did a big mistake
» against Gho, so they have been forced - indirect - to “confirm” Gho’s
» successful technique, because it definitely WORKS and that has been proven
» already!
»
» He he, it seems they all (Docs) have just disgraced themselves with their
» attacks (and penitently signatures after that) against Dr. Gho … :smiley:
»
» I would love to see their faces during their signature act. :smiley:
»
» By the way - Here you can READ (translate) what happened:
» http://www.nieuwsbank.nl/inp/2010/01/22/G002.htm


#12

» » » http://www.freepatentsonline.com/y2010/0034856.html
» »
» » More Gho bullsht. How does it go again? “Fool me once, shame on you.
» » Fool me twice, shame on me.”
»
» Heres a link to a journal and their website by them - better photos than
» they used to show,but I’m still not convinced
»
» Oh sh*t Amsterdam would be very easy for me to get to as well - Last time
» I went there a good looking woman asked me do I have the time - I looked at
» my watch stupid me (Amsterdam is well known for prositutes) - The amount of
» beggers on the street,was a lot,to.It was as if they knew you was a tourist
» - Loads of McDonalds,to - horrible place
»
» http://www.dewittevrouwenhof.nl/algemeen/layout_images/brochures/hsi_haarjournaal_08_uk.pdf
»
» Heres their website
»
» http://www.hasci.com/Default.aspx

Dr Gho = A big nothing,its a FUE procedure,always as been,theres never been any proof that donor hair re-grows.If you read about procedure,doesn’t it really sound just like FUE


#13

» » If you read about procedure,doesn’t
» it really sound just like FUE

Correct.

It is very SIMILAR to FUE technique (as described within the patent obove), but with the help of regenerativ medicine - and “in vivo”.

With other words:
It is the same technique, which currently Dr. Jerry Cooley (North Carolina, US) or Dr. Hitzig (New York, US) try to accomplish with ACell’s Extra Celluar Matrix (ECM) MatriStem and/or in combination with some other growth factors.

By the way - Call this technique/method “classic hairmultiplication”. Because this technique has nothing to do with methods/therapies like Aderans, Histogen or Follica seem to already have accomplished. Because they have solutions for “in vitro”.


#14

» Dr Gho = A big nothing,its a FUE procedure,always as been,theres never
» been any proof that donor hair re-grows.If you read about procedure,doesn’t
» it really sound just like FUE

By the way - I already posted something about your “nothing” yesterday:
http://www.hairsite.com/hair-loss/forum_entry-id-63683-page-0-category-1-order-last_answer.html

There you can find the answer about the WHY “never been any proof …”


#15

» Wow, this is really starting to sound like they might be on to
» something…nothing… this already exists to the many
» years, and nobody showed swims, no photo, no image, nothing, nothing,
» nothing, doctor GHO= NOTHING…

no photos? haven’t you been to his homepage? its full of photos…


#16

» » … this already exists to the many
» » years, and nobody showed swims, no photo, no image, nothing, nothing,
» » nothing, doctor GHO= NOTHING
»
» Why “nothing”? What do you expect?
»
» Go to the “NEUWS” section on this site:
» http://www.hasci.com/default.aspx
»
» There you will find a VIDEO about this process & technique,
» patented and practiced by Dr. Gho.
» Furthermore, look at the first (start) post above. There (patent) you can
» READ in English, HOW and WHY his technique works.
»
» It seems, some antagonistic Docs out there, already did a big mistake
» against Gho, so they have been forced - indirect - to “confirm” Gho’s
» successful technique, because it definitely WORKS and that has been proven
» already!
»
» He he, it seems they all (Docs) have just disgraced themselves with their
» attacks (and penitently signatures after that) against Dr. Gho … :smiley:
»
» I would love to see their faces during their signature act. :smiley:
»
» By the way - Here you can READ (translate) what happened:
» http://www.nieuwsbank.nl/inp/2010/01/22/G002.htm

umm… sorry but from the link you sent me it sounds more like those docs signed to WARN people about GHO’s clinic. Not to endorse him.


#17

» » » http://www.freepatentsonline.com/y2010/0034856.html
» »
» » More Gho bullsht. How does it go again? “Fool me once, shame on you.
» » Fool me twice, shame on me.”
»
» Heres a link to a journal and their website by them - better photos than
» they used to show,but I’m still not convinced
»
» Oh sh*t Amsterdam would be very easy for me to get to as well - Last time
» I went there a good looking woman asked me do I have the time - I looked at
» my watch stupid me (Amsterdam is well known for prositutes) - The amount of
» beggers on the street,was a lot,to.It was as if they knew you was a tourist
» - Loads of McDonalds,to - horrible place
»
» http://www.dewittevrouwenhof.nl/algemeen/layout_images/brochures/hsi_haarjournaal_08_uk.pdf
»
» Heres their website
»
» http://www.hasci.com/Default.aspx

I would think that if this technique actually works, every HT surgeon would know of it, and be looking into learning the technique. This is essentially the best case scenario for HT surgeons, as it allow for graft placement without depleting the donor supply.


#18

Agreed.

If slapping something like Acell on the donor wounds would regrow the lost donor hair, then the HT industry will have every reason to embrace this as quickly (and loudly) as they can make it happen.


#19

» » » … this already exists to the many
» » » years, and nobody showed swims, no photo, no image, nothing, nothing,
» » » nothing, doctor GHO= NOTHING
» »
» » Why “nothing”? What do you expect?
» »
» » Go to the “NEUWS” section on this site:
» » http://www.hasci.com/default.aspx
» »
» » There you will find a VIDEO about this process & technique,
» » patented and practiced by Dr. Gho.
» » Furthermore, look at the first (start) post above. There (patent) you
» can
» » READ in English, HOW and WHY his technique works.
» »
» » It seems, some antagonistic Docs out there, already did a big mistake
» » against Gho, so they have been forced - indirect - to “confirm” Gho’s
» » successful technique, because it definitely WORKS and that has been
» proven
» » already!
» »
» » He he, it seems they all (Docs) have just disgraced themselves with
» their
» » attacks (and penitently signatures after that) against Dr. Gho … :smiley:
» »
» » I would love to see their faces during their signature act. :smiley:
» »
» » By the way - Here you can READ (translate) what happened:
» » http://www.nieuwsbank.nl/inp/2010/01/22/G002.htm
»
» umm… sorry but from the link you sent me it sounds more like those docs
» signed to WARN people about GHO’s clinic. Not to endorse him.

Hairman - I think Ironman’s initial post was not clear. What happened was a bunch of docs wrote an article discrediting Gho’s technique. The article is found here:

Thereafter, Gho’s clinic took action and (I presume) proved that his hair multiplication technique worked. Those same doctors then had to rescind their statements, which they did in this letter:
http://www.hasci.com/uploads/downloads/Rectificatie.dr.B.Feriduni.pdf

I don’t know Dutch, so I can’t really translate, but that is my sense of what happened. Any Dutch speakers care to comment?


#20

Good work, Leonard! :slight_smile:

In fact, this is …
» What happened
» was a bunch of docs wrote an article discrediting Gho’s technique. The article
» is found here:
» http://www.nieuwsbank.nl/inp/2010/01/22/G002.htm
»
» Thereafter, Gho’s clinic took action and (I presume) proved that his hair
» multiplication technique worked. Those same doctors then had to rescind
» their statements, which they did in this letter:
» http://www.hasci.com/uploads/downloads/Rectificatie.dr.B.Feriduni.pdf

And here the current problem for these “bunch of docs”:
They are now practically UNABLE to offer/sell the same or similar HM technique to their patients, because they shouted very clear and loud from the roofs “Hair Multiplication (2 hairs from 1) do not work! Dr. Gho is a liar!”.
After that, these “bunch of docs” have been forced by law to undersign, that hair multiplication, in fact, as practiced by Dr. Gho, WORKS. :smiley:

The truth behind all that is, that this sort of “hair multiplication” (2 or more hairs from 1) has already been proven by many scientists out there, as for example:

Dr. Kim JC in 1995 …

Dr. Gary Hitzig in 2003 …
http://www.ishrs.org/pr-pr3.htm

http://journalreview.org/v2/articles/view/7728481.html

http://www.prlog.org/10477584-researchers-develop-first-successful-hair-cloning-technique-major-advancement-in-hair-restoration.html

Dr. Pierluigi Santi and Dr. Edoardo Rapposio in 2007 …
http://www.associatedcontent.com/article/124383/good_news_for_thinning_pallets_cloning.html

And finally Dr. Gho in 2009 … :smiley:

If I would be Dr. Gho, I would let send a very nice letter by lawyers and by court to “chairman” Dr. Rassman too, because of his lie and untruth to the public … :lol3: