Objective: Current in vivo models of de novo hair growth and
regeneration used in the laboratory are arduous, unrealistic
and therefore unable to be translated to clinical practice.
We sought to improve on this by a technique that is simple,
reproducible and generates a cosmetically acceptable
appearance. Here we present a novel method in which a large
number of hair and sebaceous glands can be reliably grown
from a suspension of single cells within a matrix.
Methods: Multipotential cells were obtained from neonatal
mice using techniques from previously published work. The
cells were loaded onto a pre-shaped, pre-treated matrix. The
entire matrix, with cells loaded inside, was then grafted onto a
full thickness wound created on the dorsum of a nude mouse.
Hairs were observed for long-term growth and wound healing.
Samples of de novo hair at different stages were taken for
immunohistochemistry and PCR studies.
Results: De novo hair growing on the dorsum of a grafted
nude mouse can be seen as early as 11 days post operative.
We performed these experiments using various commercially
available and self-made biologic collagen based matrices.
Over 40 athymic nude mice have been grafted with the matrix
construct seeded with pigmented wild type multipotential
cells. Greater than 92% of mice successfully grew de novo
hair in an organized planar fashion. Immunohistochemical
studies of de novo hairs prove that hairs are real and contain
all layers of normal hair. We have followed 6 mice as they
continued to grow hair for over one year after grafting. The
hair was also tested by shaving and plucking to demonstrate
normal cycling and regeneration.
Conclusions: We have shown with high reproducibility
that cells can be grafted with the help of a scaffold-like
matrix to produce new hair in an organized and clinically
acceptable fashion. The matrix can be shaped as needed
such that this can be used for specific regions of alopecia.
Further, it is conceivable that rather than dependence on
autotransplantation, future hair restoration will be able to
use an unlimited supply of stem cells organized by a shaped
matrix.
This is cell therapy using multipotent stem cells, and scaffolds.
They say the new hairs can cycle after plucking.
But the experiment is on mice, so still no big value.
» 21: NOVEL USE OF MATRIX FOR HAIR GROWTH : Plastic and Reconstructive Surgery
»
» Objective: Current in vivo models of de novo hair growth and
» regeneration used in the laboratory are arduous, unrealistic
» and therefore unable to be translated to clinical practice.
» We sought to improve on this by a technique that is simple,
» reproducible and generates a cosmetically acceptable
» appearance. Here we present a novel method in which a large
» number of hair and sebaceous glands can be reliably grown
» from a suspension of single cells within a matrix.
»
» Methods: Multipotential cells were obtained from neonatal
» mice using techniques from previously published work. The
» cells were loaded onto a pre-shaped, pre-treated matrix. The
» entire matrix, with cells loaded inside, was then grafted onto a
» full thickness wound created on the dorsum of a nude mouse.
» Hairs were observed for long-term growth and wound healing.
» Samples of de novo hair at different stages were taken for
» immunohistochemistry and PCR studies.
»
» Results: De novo hair growing on the dorsum of a grafted
» nude mouse can be seen as early as 11 days post operative.
» We performed these experiments using various commercially
» available and self-made biologic collagen based matrices.
» Over 40 athymic nude mice have been grafted with the matrix
» construct seeded with pigmented wild type multipotential
» cells. Greater than 92% of mice successfully grew de novo
» hair in an organized planar fashion. Immunohistochemical
» studies of de novo hairs prove that hairs are real and contain
» all layers of normal hair. We have followed 6 mice as they
» continued to grow hair for over one year after grafting. The
» hair was also tested by shaving and plucking to demonstrate
» normal cycling and regeneration.
»
» Conclusions: We have shown with high reproducibility
» that cells can be grafted with the help of a scaffold-like
» matrix to produce new hair in an organized and clinically
» acceptable fashion. The matrix can be shaped as needed
» such that this can be used for specific regions of alopecia.
» Further, it is conceivable that rather than dependence on
» autotransplantation, future hair restoration will be able to
» use an unlimited supply of stem cells organized by a shaped
» matrix.
» This is cell therapy using multipotent stem cells, and scaffolds.
» They say the new hairs can cycle after plucking.
» But the experiment is on mice, so still no big value.
»
»
»
» »
» 21: NOVEL USE OF MATRIX FOR HAIR GROWTH : Plastic and Reconstructive Surgery
» »
» » Objective: Current in vivo models of de novo hair growth and
» » regeneration used in the laboratory are arduous, unrealistic
» » and therefore unable to be translated to clinical practice.
» » We sought to improve on this by a technique that is simple,
» » reproducible and generates a cosmetically acceptable
» » appearance. Here we present a novel method in which a large
» » number of hair and sebaceous glands can be reliably grown
» » from a suspension of single cells within a matrix.
» »
» » Methods: Multipotential cells were obtained from neonatal
» » mice using techniques from previously published work. The
» » cells were loaded onto a pre-shaped, pre-treated matrix. The
» » entire matrix, with cells loaded inside, was then grafted onto a
» » full thickness wound created on the dorsum of a nude mouse.
» » Hairs were observed for long-term growth and wound healing.
» » Samples of de novo hair at different stages were taken for
» » immunohistochemistry and PCR studies.
» »
» » Results: De novo hair growing on the dorsum of a grafted
» » nude mouse can be seen as early as 11 days post operative.
» » We performed these experiments using various commercially
» » available and self-made biologic collagen based matrices.
» » Over 40 athymic nude mice have been grafted with the matrix
» » construct seeded with pigmented wild type multipotential
» » cells. Greater than 92% of mice successfully grew de novo
» » hair in an organized planar fashion. Immunohistochemical
» » studies of de novo hairs prove that hairs are real and contain
» » all layers of normal hair. We have followed 6 mice as they
» » continued to grow hair for over one year after grafting. The
» » hair was also tested by shaving and plucking to demonstrate
» » normal cycling and regeneration.
» »
» » Conclusions: We have shown with high reproducibility
» » that cells can be grafted with the help of a scaffold-like
» » matrix to produce new hair in an organized and clinically
» » acceptable fashion. The matrix can be shaped as needed
» » such that this can be used for specific regions of alopecia.
» » Further, it is conceivable that rather than dependence on
» » autotransplantation, future hair restoration will be able to
» » use an unlimited supply of stem cells organized by a shaped
» » matrix.
I really find the area of usin scaffolds really facinating. it has been used to wonderful effect with human tracheas and is something i’d love to see carried out in humans with donor hair follicles
» » This is cell therapy using multipotent stem cells, and scaffolds.
» » They say the new hairs can cycle after plucking.
» » But the experiment is on mice, so still no big value.
» »
» »
» »
» » »
» »
» 21: NOVEL USE OF MATRIX FOR HAIR GROWTH : Plastic and Reconstructive Surgery
» » »
» » » Objective: Current in vivo models of de novo hair growth and
» » » regeneration used in the laboratory are arduous, unrealistic
» » » and therefore unable to be translated to clinical practice.
» » » We sought to improve on this by a technique that is simple,
» » » reproducible and generates a cosmetically acceptable
» » » appearance. Here we present a novel method in which a large
» » » number of hair and sebaceous glands can be reliably grown
» » » from a suspension of single cells within a matrix.
» » »
» » » Methods: Multipotential cells were obtained from neonatal
» » » mice using techniques from previously published work. The
» » » cells were loaded onto a pre-shaped, pre-treated matrix. The
» » » entire matrix, with cells loaded inside, was then grafted onto a
» » » full thickness wound created on the dorsum of a nude mouse.
» » » Hairs were observed for long-term growth and wound healing.
» » » Samples of de novo hair at different stages were taken for
» » » immunohistochemistry and PCR studies.
» » »
» » » Results: De novo hair growing on the dorsum of a grafted
» » » nude mouse can be seen as early as 11 days post operative.
» » » We performed these experiments using various commercially
» » » available and self-made biologic collagen based matrices.
» » » Over 40 athymic nude mice have been grafted with the matrix
» » » construct seeded with pigmented wild type multipotential
» » » cells. Greater than 92% of mice successfully grew de novo
» » » hair in an organized planar fashion. Immunohistochemical
» » » studies of de novo hairs prove that hairs are real and contain
» » » all layers of normal hair. We have followed 6 mice as they
» » » continued to grow hair for over one year after grafting. The
» » » hair was also tested by shaving and plucking to demonstrate
» » » normal cycling and regeneration.
» » »
» » » Conclusions: We have shown with high reproducibility
» » » that cells can be grafted with the help of a scaffold-like
» » » matrix to produce new hair in an organized and clinically
» » » acceptable fashion. The matrix can be shaped as needed
» » » such that this can be used for specific regions of alopecia.
» » » Further, it is conceivable that rather than dependence on
» » » autotransplantation, future hair restoration will be able to
» » » use an unlimited supply of stem cells organized by a shaped
» » » matrix.
»
» I really find the area of usin scaffolds really facinating. it has been
» used to wonderful effect with human tracheas and is something i’d love to
» see carried out in humans with donor hair follicles
Is this similar to what Cooley/Hitzig are doing with Acell? (ie: cycling plucked hairs)?
Can someone specify for me also what exactly a “matrix” is? Is it like a piece of skin (in physical terms)?
» » nude mouse
» »
» »
» » Thank’s for the news… but read again in 2010 about NUDE MOUSEs is
» very
» » sad.
»
» i hope you keep on updating your blog
»
» Cause there are some news you didnt cover
» » » nude mouse
» » »
» » »
» » » Thank’s for the news… but read again in 2010 about NUDE MOUSEs is
» » very
» » » sad.
» »
» » i hope you keep on updating your blog
» »
» » Cause there are some news you didnt cover
»
» my blog is dead.
Why? There is no use for this cause we got a lot of infos and stuff.
If you dont want to keep it going, i would gladly do it
» » » » nude mouse
» » » »
» » » »
» » » » Thank’s for the news… but read again in 2010 about NUDE MOUSEs is
» » » very
» » » » sad.
» » »
» » » i hope you keep on updating your blog
» » »
» » » Cause there are some news you didnt cover
» »
» » my blog is dead.
»
» Why? There is no use for this cause we got a lot of infos and stuff.
»
» If you dont want to keep it going, i would gladly do it
it would be really interesting to see a small trial carried out in humans to assess this.
» » » » » nude mouse
» » » » »
» » » » »
» » » » » Thank’s for the news… but read again in 2010 about NUDE MOUSEs
» is
» » » » very
» » » » » sad.
» » » »
» » » » i hope you keep on updating your blog
» » » »
» » » » Cause there are some news you didnt cover
» » »
» » » my blog is dead.
» »
» » Why? There is no use for this cause we got a lot of infos and stuff.
» »
» » If you dont want to keep it going, i would gladly do it
»
» it would be really interesting to see a small trial carried out in humans
» to assess this.
After a year of testing with much success, when will the human trials begin? I know there is no shortage of men who would gladly particpate in this trial. Atleast try it on one person. Either they don’t give all the details(abnormal growths) or they exaggerate!
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