http://www.nature.com/jid/journal/vaop/naam/pdf/jid2013337a.pdf
looks like good old George Cotsarelis is still alive and kicking…
Rajesh L Thangapazham, Peter Klover, Ji-an Wang, Ying Zheng, Amanda Devine, Shaowei Li, Leonard Sperling, George Cotsarelis and Thomas N Darling
Another new paper… but this time from a Japanese company called Phoenix Bio
“Contact Between Dermal Papilla Cells and Dermal Sheath Cells Enhances the Ability of DPCs to Induce Hair Growth.”
We previously showed that cultured rat dermal papilla cells (DPCs) retain their hair-inducing capacity on afollicular epidermal cell (EPCs). Here, we examined the hair growth-inducing capacity of differently subcultured DPCs by transplanting them, along with rat EPCs, onto the backs of nude mice (graft chamber assay). DPCs at passage § 30) (high-passage DPCs) had no such activity and induced only subepidermal hair follicles (HFs) that were not encapsulated by the dermal sheath (DS). Thus, we examined the effect of DS cells (DSCs(p=1)) on the ability of DPCs(p=60) to induce hair growth by testing a mixture of these two cell types (cotransplant) in the graft chamber assay, in which DSCs(p=1) and DPCs(p=60) were labeled with enhanced green fluorescent protein (EGFP) and 1,1-dioctadecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate (DiI), respectively. These cotransplants generated hairs as actively as did DPCs(p=6) transplants. Their HFs were encapsulated with EGFP(+)-DS and had DPs consisting largely of EGFP(+)-DPCs (47%) and DiI(+)-DPCs (43%), indicating a major contribution of DSC(p=1)-derived DPCs to HF induction. In addition, the results of in vitro coculture of DPCs(p=60) and DSCs(p=1) suggest that high-passage DPCs stimulate the expression of certain trichogenic genes in DSCs.Journal of Investigative Dermatology advance online publication, 19 August 2010; doi:10.1038/jid.2010.241.
Affiliation
PhoenixBio, Higashihiroshima, Japan.
Journal Details
This article was published in the following journal.
Name: The Journal of investigative dermatology
ISSN: 1523-1747
Pages:
This one is new? It looks like the date is 19 August 2010.
Anyone have a link to the full paper?
Would be an interesting read if we could get around the paywall.