Better Ideas than <..>

Stem cell; cell based hair therapy
21

[quote][postedby]Originally Posted by roger_that[/postedby]
Yes, i think you’re right, jarjar. SB did not disclose their full blueprint for converting pluripotent stem cells into DP cells in their paper, at least that’s the way it looked to me. But someone could read that paper and now that they know it’s doable, a smart scientist could probably fill in the gaps and reconstruct what SB did.[/quote]

I don’t think so. Science is not easy. And until some “smart scientist” does this all we know is that it has no chance of becoming available because SB is not sharing its’ information. So we should be looking at other possibilities even if it’s highly unlikely that the other possibilities will work because at least those other possibilities are moving forward whereas SB is stuck in a ditch indefinitely.

22

But they’re not stuck in a ditch indefinitely. They’re just not announcing every little thing they do, so to some people it may look like nothing’s happening.

23

[quote][postedby]Originally Posted by roger_that[/postedby]
But they’re not stuck in a ditch indefinitely. They’re just not announcing every little thing they do, so to some people it may look like nothing’s happening.[/quote]

Chances are they already filed a patent. It’s their model. Research and File IP. Then if they stumble on something, commercialize it with a partner.

We’ll never know if they did file until it is published.

24

Patent applications don’t have to be disclosed, but can be made public in the USPTO database at the request of the applicant.

Checking the USPTO database for patent applications, I see nothing from SB yet but Drs. Lindner and Lauster filed an application on December 18, 2014 for “Methods for Producing Hair Microfollicles and De Novo Papillae and Their Use for In Vitro Tests and In Vivo Implantations” –

http://appft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=5&f=G&l=50&co1=AND&d=PG01&s1=cotsarelis&s2=hair&OS=cotsarelis+AND+hair&RS=cotsarelis+AND+hair

25

How you like me now

Lasers Surg Med. 2015 May 6. doi: 10.1002/lsm.22358. [Epub ahead of print]
Hair regrowth through wound healing process after ablative fractional laser treatment in a murine model.
Bae JM1, Jung HM, Goo B, Park YM.
Author information
Abstract
BACKGROUND AND OBJECTIVE:
Alopecia is one of the most common dermatological problems in the elderly; however, current therapies for it are limited by low efficacy and undesirable side effects. Although clinical reports on fractional laser treatment for various alopecia types are increasing, the exact mechanism remains to be clarified. The purposes of this study were to demonstrate the effect of ablative fractional laser treatment on hair follicle regrowth in vivo and investigate the molecular mechanism after laser treatment.
MATERIALS AND METHODS:
Ablative CO2 fractional laser was applied to the shaved dorsal skin of 7-week-old C57BL/6 mice whose hair was in the telogen stage. After 12 mice were treated at various energy (10-40 mJ/spot) and density (100-400 spots/cm2 ) settings to determine the proper dosage for maximal effect. Six mice were then treated at the decided dosage and skin specimens were sequentially obtained by excision biopsy from the dorsal aspect of each mouse. Tissue samples were used for the immunohistochemistry and reverse transcription polymerase chain reaction assays to examine hair follicle status and their related molecules.
RESULTS:
The most effective dosage was the 10 mJ/spot and 300 spots/cm2 setting. The anagen conversion of hair was observed in the histopathological examination, while Wnt/β-catenin expression was associated with hair regrowth in the immunohistochemistry and molecular studies.
CONCLUSIONS:
Ablative fractional lasers appear to be effective for inducing hair regrowth via activation of the Wnt/β-catenin pathway in vivo. Our findings indicate that fractional laser treatment can potentially be developed as new treatment options for stimulating hair regrowth. Lasers Surg. Med. © 2015 Wiley Periodicals, Inc.
© 2015 Wiley Periodicals, Inc.
KEYWORDS:
alopecia; androgenic alopecia; fractional laser; hair follicle; hair loss; laser

26

[quote][postedby]Originally Posted by needhairasap[/postedby]
How you like me now

Lasers Surg Med. 2015 May 6. doi: 10.1002/lsm.22358. [Epub ahead of print]
Hair regrowth through wound healing process after ablative fractional laser treatment in a murine model.
Bae JM1, Jung HM, Goo B, Park YM.
Author information
Abstract
BACKGROUND AND OBJECTIVE:
Alopecia is one of the most common dermatological problems in the elderly; however, current therapies for it are limited by low efficacy and undesirable side effects. Although clinical reports on fractional laser treatment for various alopecia types are increasing, the exact mechanism remains to be clarified. The purposes of this study were to demonstrate the effect of ablative fractional laser treatment on hair follicle regrowth in vivo and investigate the molecular mechanism after laser treatment.
MATERIALS AND METHODS:
Ablative CO2 fractional laser was applied to the shaved dorsal skin of 7-week-old C57BL/6 mice whose hair was in the telogen stage. After 12 mice were treated at various energy (10-40 mJ/spot) and density (100-400 spots/cm2 ) settings to determine the proper dosage for maximal effect. Six mice were then treated at the decided dosage and skin specimens were sequentially obtained by excision biopsy from the dorsal aspect of each mouse. Tissue samples were used for the immunohistochemistry and reverse transcription polymerase chain reaction assays to examine hair follicle status and their related molecules.
RESULTS:
The most effective dosage was the 10 mJ/spot and 300 spots/cm2 setting. The anagen conversion of hair was observed in the histopathological examination, while Wnt/β-catenin expression was associated with hair regrowth in the immunohistochemistry and molecular studies.
CONCLUSIONS:
Ablative fractional lasers appear to be effective for inducing hair regrowth via activation of the Wnt/β-catenin pathway in vivo. Our findings indicate that fractional laser treatment can potentially be developed as new treatment options for stimulating hair regrowth. Lasers Surg. Med. © 2015 Wiley Periodicals, Inc.
© 2015 Wiley Periodicals, Inc.
KEYWORDS:
alopecia; androgenic alopecia; fractional laser; hair follicle; hair loss; laser[/quote]

I have a really hard time getting excited about this. The report talks about “the most effective dosage” and “hair growth” but provides zero details about how much hair growth. Come on, let’s be realistic. If there was really impressive hair growth, we’d have a much more detailed report in this summary with hair counts and photos. Plus, this was done on mice (again) and as we know, most of what is possible for mice in terms of hair regrowth does NOT translate well to humans.

Next…

27

please, not another study on mice.

28

Roger has a “hard time” getting excited about it. I can not get excited about it period.

29

[quote][postedby]Originally Posted by needhairasap[/postedby]
How you like me now

Lasers Surg Med. 2015 May 6. doi: 10.1002/lsm.22358. [Epub ahead of print]
Hair regrowth through wound healing process after ablative fractional laser treatment in a murine model.
Bae JM1, Jung HM, Goo B, Park YM.
Author information
Abstract
BACKGROUND AND OBJECTIVE:
Alopecia is one of the most common dermatological problems in the elderly; however, current therapies for it are limited by low efficacy and undesirable side effects. Although clinical reports on fractional laser treatment for various alopecia types are increasing, the exact mechanism remains to be clarified. The purposes of this study were to demonstrate the effect of ablative fractional laser treatment on hair follicle regrowth in vivo and investigate the molecular mechanism after laser treatment.
MATERIALS AND METHODS:
Ablative CO2 fractional laser was applied to the shaved dorsal skin of 7-week-old C57BL/6 mice whose hair was in the telogen stage. After 12 mice were treated at various energy (10-40 mJ/spot) and density (100-400 spots/cm2 ) settings to determine the proper dosage for maximal effect. Six mice were then treated at the decided dosage and skin specimens were sequentially obtained by excision biopsy from the dorsal aspect of each mouse. Tissue samples were used for the immunohistochemistry and reverse transcription polymerase chain reaction assays to examine hair follicle status and their related molecules.
RESULTS:
The most effective dosage was the 10 mJ/spot and 300 spots/cm2 setting. The anagen conversion of hair was observed in the histopathological examination, while Wnt/β-catenin expression was associated with hair regrowth in the immunohistochemistry and molecular studies.
CONCLUSIONS:
Ablative fractional lasers appear to be effective for inducing hair regrowth via activation of the Wnt/β-catenin pathway in vivo. Our findings indicate that fractional laser treatment can potentially be developed as new treatment options for stimulating hair regrowth. Lasers Surg. Med. © 2015 Wiley Periodicals, Inc.
© 2015 Wiley Periodicals, Inc.
KEYWORDS:
alopecia; androgenic alopecia; fractional laser; hair follicle; hair loss; laser

[postedby]Originally Posted by roger_that[/postedby]

I have a really hard time getting excited about this. The report talks about “the most effective dosage” and “hair growth” but provides zero details about how much hair growth. Come on, let’s be realistic. If there was really impressive hair growth, we’d have a much more detailed report in this summary with hair counts and photos. Plus, this was done on mice (again) and as we know, most of what is possible for mice in terms of hair regrowth does NOT translate well to humans.

Next…[/quote]

Okay roger…

  1. The work SB’s research started from is based on mice…so apparently it can translate over with tweaks… additionally minox and fin grow hair on mice and men… again… it can translate over… obviously not everything does (such an obvious point it’s not really worth mentioning, but whatever)

  2. IN the other study I posted, which you didn’t read, they got 100 regrowth in the treated areas… so…

anyway, some news for roger:

Tissue Eng Part C Methods. 2015 May 7. [Epub ahead of print]
A two-stepped culture method for efficient production of trichogenic keratinocytes.
Chan CC1, Fan MY, Wang WH, Mu YF, Lin SJ.
Author information
Abstract
Successful hair follicle neogenesis in adult life depends on existence of both capable dermal cells and competent epidermal keratinocytes that recapitulate embryonic organogenesis through epithelial-mesenchymal interaction. In tissue engineering, the maintenance of trichogenic potential of adult epidermal cells while expanding them remains a challenging issue. We found that though hair follicle outer root sheath keratinocytes could be expanded for more than 100 passages as clonogenic cells without losing the proliferative potential with a 3T3J2 fibroblast feeder layer, these keratinocytes were unable to form new hair follicles when combined with inductive hair follicle dermal papilla cells. However, when these high passage keratinocytes were co-cultured with hair follicle dermal papilla cells for 4 days in vitro, they regained the trichogenic ability to form new hair follicles after transplantation. We found that the short-term co-culture with dermal papilla cells both enhanced Wnt/β-catenin signaling, a signaling cascade key to hair follicle development, and upregulated the expression of hair follicle specific genes, including K6, K16, K17 and K75, in keratinocytes, indicating these cells were poised toward a hair follicle fate. Hence, efficient production of trichogenic keratinocytes can be obtained by a two-stepped procedure with initial cell expansion with a 3T3J2 fibroblast feeder followed by short-term co-culture with dermal papilla cells.
PMID: 25951188 [PubMed - as supplied by publisher]